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recombinant antigen gene 复合抗原基因
Keywords Toxoplasma gondii;multiepitope antigenic gene;recombinant antigen;immunodiagnosis;ELISA; 弓形虫;多表位基因;重组抗原;免疫诊断;ELISA试剂盒;
Keywords Toxoplasma gondii;multiepitope antigenic gene;recombinant antigen;vaccine;immunodiagnosis;ELISA kit; 弓形虫;多表位基因;重组抗原;疫苗;免疫诊断;ELISA试剂盒;
Because of high sensitivity and specificity,purified antigen and recombinant antigen may be used to diagnose schistosomiasis. 纯化抗原和重组抗原因敏感性高,特异性强,有望解决血吸虫病的诊断问题。
Objective Cloning of 14-3-3 antigen gene of Schistosoma japonicum in order to its study for immunological diagnosis and prevention. 目的克隆日本血吸虫14-3-3抗原的编码基因,以研究其用于血吸虫病免疫诊断和免疫预防效果。
The combined PCX/S antigen gene could induce specific immune response and it might be able to serve as a bivalent DNA vaccines candidate specific for HCV and HBV. 复合型PCX/S抗原基因可诱发特异性免疫应答,为HCV/HBV双价疫苗的研究提供一定实验基础。
By Western-blotting and ELISA it was shown that thesefour McAb could react with three kinds of recombinant antigen, pcDNA3/MDR 1,pcDNA3/P-gp and pHVaMDR 1. They create advantaged condition for clinic test. 通过Western-blotting试验和间接ELISA法检测证明4株McAb可与pcDNA3/MDR 1、pcDNA3/P-gp和pHVaMDR 1 3种重组抗原反应。为临床检测MDR 1抗原创造有利条件。
Methods The specific PCR primers were designed and synthesized to amplify the DNA fragments enconding preS1/S2/S antigen gene from the plasmid pHBV adr by PCR method. 方法设计合成2对寡核苷酸引物,以adr亚型HBV质粒pHBV DNA为模板,采用PCR法分别扩增HBV大分子表面蛋白基因(preS1/S2/S基因)片段;
Keywords Treponema pallidum;Gene expression;Recombinant antigen; 梅毒螺旋体;基因表达;重组抗原;
E. granulosus ferritin gene gained from protoscolexes can be used as candidate antigen gene to develop vaccine and further biological functions studying. 结论测序的铁蛋白基因序列有完整的编码框，为进一步研究其结构和功能，以及对包虫病的免疫预防具有重要意义。
Conclusion The full length variant-specific surface antigen gene from the isolate of G. lamblia has the common characteristics with other previously identified VSPs. 结论中国人源蓝氏贾第鞭毛虫滋养体表达的表面变异抗原基因具有与已鉴定的蓝氏贾第鞭毛虫表面变异抗原基因同样的特性。
The limited source of native antigens restricts its use.The application of recombinant antigens will solve the problems of antigen source and quality control. 天然抗原来源有限,限制了其应用,而重组抗原的应用可解决质量控制和抗原来源的问题。
As yet, there is no national data about human rotavirus antigen gene expressed in transgenic plants although there are reports of murine (epitope of NSP4) or bovine (VP6) rotavirus (Matsumura et al., 2002; Yu J et al., 2001). Wang等人1999年将猪源轮状病毒VP7基因改造后在大肠杆菌中表达，也具有很好的中和病毒能力，何金生等人2001年以腺病毒表达人源轮状病毒VP7免疫小鼠，产生较强的体液和粘膜免疫应答，次年彭世勇等人用pCI分别进行VP7、VP4和VP6基因的DNA疫苗研究，结果仅VP7诱导最强的免疫应答。
The sensitivity and specificity of the recombinant antigens used in ELISA (rSj-ELISA) were compared in parallel with the traditional methods of SEA-IHA and COPT. 同时比较3种方法在华支睾吸虫和钩虫感染者之间的交叉反应性，以探讨重组抗原用于诊断血吸虫病的实用性和可靠性。
It is a new approach to study and produce HCV vaccine by using multi-epitope antigen of HCV. In this study, multi-epitope antigen gene, PCX, which consists of 5 epitopes of HCV and an epitope of T cell stimulation o f tetanus toxoid, was expressed in E. 利用HCV抗原多表位来研制HCV疫苗是目前的一个新方向。
In this paper, the research in optimization design for DNA vaccine was reviewed, including choice of antigen gene and immune adjuvant, optimization of plasmid vectors and inoculation methods. 许多试图增强和调节质粒DNA疫苗的免疫原性和效率的方案都在研究之中。
Methods HEV antibodies in serum samples obtained from swine,sleuth,domestic dog,pet dog,goat,chicken,duck,pigeon and rabbit were tested by a double-antigen sandwich ELISA(DS-ELISA)which was established using HEV multiple-genotype recombinant antigens. 方法用多基因型HEV重组蛋白建立检测HEV抗体的DS -ELISA ,并用于 5 91份猪、警犬、家犬、宠物犬、山羊、鸡、鸭、鸽子和兔等多种动物血清标本的HEV抗体检测 ;
Detection of anti-SSB autoantibody using recombinant antigen 利用重组抗原检测抗SSB自身抗体及其临床意义
Keywords hepatitis delta virus;recombinant antigen;HDAg; 关键词丁型肝炎病毒;重组抗原;丁肝抗原;