Methods The specific PCR primers were designed and synthesized to amplify the DNA fragments enconding preS1/S2/S antigen gene from the plasmid pHBV adr by PCR method.

• 方法设计合成2对寡核苷酸引物,以adr亚型HBV质粒pHBV DNA为模板,采用PCR法分别扩增HBV大分子表面蛋白基因(preS1/S2/S基因)片段;