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Both genes were cloned into GFP expression vector pIRES2 EGFP and transfected into HeLa cells. 将上述基因克隆入绿色荧光蛋白(GFP)表达载体pIRES2-EGFP中,转染人HeLa细胞,在荧光显微镜和电镜下观察转染细胞的形态和结构。
EGFP and TK were successfully fused and cloned into pBABE-puro-lox to construct a new vector called pBGTKlox. 成功拼接EGFP-TK融合基因并克隆入pBABE-puro-lox,构建成新栽体pBGTKlox。
The percentage of lung metastases in hEndo group, EGFP group and PBS group was 0.0%, 50.0% and 66.7% respectively. HENDO组、EGFP组、PBS组肺转移率分别为0·0%25、50·0%25、66·7%25;
ALR gene was inserted into pEGFP-Cl plasmid to construct ALR and EGFP co-expression vector pEGFP/ALR. ALR和EGFP融合基因表达载体，pEGFP/ALR。
Results1. After selection by culturing with G418, clones of transgenic hMSCs were obtained and showed bright EGFP expression. 感染后的hMSCs经G418筛选后逐渐形成克隆,克隆中的hMSCs均表达EGFP,在倒置荧光显微镜下可见明亮的绿色荧光。
ALR gene was also inserted to bicistronic plasmid p! RES2-EGFP to produce ALR, EGFP gene bicistronic eukaryotic expression vector (pIRES-EGFP/ALR). ALR和EGFP基因双顺反子表达载体，pIRES-EGFP/ALR。
The transfection and expression efficiency of RV was above 30% in SK-N-SH cells determined by observing EGFP expression. 在SK-N-SH细胞EGFP的荧光表达显示RV的转移并且表达的效率达到30%25以上。
The enhanced green fluorescent protein(EGFP) plasmid was used as positive control and the transfection efficiency was detected by flow cytometry. 同时转染带有增强型绿色荧光蛋白(EGFP)的载体作为阳性对照,流式细胞术检测其绿色荧光以确定转染效率;
Meanwhile, localization of EGFP within electroporated schistosomula was performed with confocal laser scanning microscope. 同时,使用激光共聚焦扫描显微镜对EGFP在童虫体内进行定位。
Aim:To study the dynamic expression of green fluorescent protein (GFP) and enhanced green fluorescent protein (EGFP) on C6 glioblastoma cells in vivo. 目的:研究绿色荧光蛋白报道基因体外转染C6胶质瘤细胞系的动态表达。
For subcellular localization, TBX5 was expressed as an EGFP fusion protein in rat hepatocarcinoma cells.Results showed that TBX5 was nuclear. 构建荧光表达载体,转染大鼠肝癌细胞,观察TBX5亚细胞定位,结果证实TBX5基因在细胞核中表达;
Objective: To express and purify fusion protein from angiogenin(ANG) and enhanced green fluorescent protein (EGFP) to further study the function of angiogenin. 目的:表达并纯化血管生成素(ANG)与增强型绿色荧光蛋白(EGFP)融合蛋白,以进一步探讨血管生成素的生物学功能。
The genetically modified LoVo/GFP cells gave off strikingly bright green fluorescence, in which the integration of EGFP provirus was confirmed by PCR analysis. 双嗜型EGFP转导的LoVo细胞可稳定发出明亮的绿色荧光信号。 PCR分析显示 ,LoVo/GFP细胞内整合有EGFP原病毒。
Methods Using plasmid as template,full-length EGFP gene was amplified. The amplified EGFP gene and pET28a vector were cleaved by EcoR I and Hind III,then ligated after purifying. 方法以质粒pEGFP-N1为模板扩增EGFP基因,EcoR I和Hind III双酶切EGFP基因序列和pET28a载体。
After the plasmid EGFP-C1 was transfected into INPC, the stable cell clone designated INPC/EGFP was isolated by G418 selection. The positive rate of EGFP was observed. 质粒EGFP-C1转染INPC后;经G418筛选;挑选细胞克隆;命名为INPC/EGFP;观察其EGFP阳性率.
Methods:Prostate cancer cells PC-3 and 22Rvl were transfect- ed with plasmid pEGFP-C1 coated with chitosan nanoparticles and the expression of EGFP was observed. 方法:将壳聚糖纳米粒包裹的含报告基因的质粒pEGFP-C1分别转染前列腺癌细胞PC-3和22Rv1,并观察EGFP的表达情况。
Objective In order to observe the infiltration and metastasis of the tumor,establish a high-metastatic lung cancer cell line expressing enhanced green fluorescent protein(EGFP). 目的建立能稳定高表达增强型绿色荧光蛋白(EGFP)的肺癌细胞株,为研究肺癌的浸润与转移机制打下基础。
Fluorescence of EGFP was localized in tegument and subtegument of the electroporated schisto?鄄somula with confocal microscopy, especially in the anterior part of the worm. 激光共聚焦显微镜观察表明EGFP主要定位在童虫的皮层和副皮层,虫体前端尤为明显。
Ultrasound mediated microbubble destruction at 0.8 MHz , 1.0 W/cm 2, 10% duty cycle, 60 s can get the most stable effective expression of EGFP gene in Cos 7 cell and no cytotoxicity. 体外试验发现 0 .;8MHz、1
EGFP alone failed to transduce into human aortic smooth muscle cells, whereas PEP-1-EGFP fusion protein could transduce into them and distributed in cytoplasm and nucleus after 1h incubation. EGFP蛋白不能进入细胞内,PEP-1-EGFP融合蛋白和人平滑肌细胞共同孵育1h后可见有明亮绿色荧光分布于胞浆和胞核。