For subcellular localization, TBX5 was expressed as an EGFP fusion protein in rat hepatocarcinoma cells.Results showed that TBX5 was nuclear.

• 构建荧光表达载体,转染大鼠肝癌细胞,观察TBX5亚细胞定位,结果证实TBX5基因在细胞核中表达;