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oligodeoxynucleotide antisense 反义寡脱氧核糖核苷酸
The antisense drug synthesized by DNA synthesizer is an oligodeoxynucleotide (ODN). 由机器合成的反义核酸药物需要有效的纯度鉴定方法。
Antisense oligodeoxynucleotide technique was used to observe the role of CLC-3 in the effect of ET-1 on BCECs. 采用反义核酸技术，观察CLC-3基因在ET-1对体外培养牛角膜内皮细胞影响中的作用。
OPC colum provides a high effciency,quick and convenient method for the purification of antisense phosphorothioate Oligodeoxynucleotide to Bcl 2. Bcl- 2硫代反义核酸 OPC纯化法是一种高效、快速、简便的方法
To study the effect of antisense focal adhesion kinase (FAK) oligodeoxynucleotide on the biological behaviors of U251 MG cells. 目的探讨反义黏着斑激酶(FAK)对U251人胶质瘤细胞株生物学行为影响。
Objective To study the effect of antisense oligodeoxynucleotide c-myc (c-myc ASODN) on anti-oxidation system in LEC induced by galactose. 目的探讨ASODN对半乳糖性白内障晶体上皮细胞(LEC)中抗氧化系统活力的影响。
Aim: To observe if bcl-2 antisense oligodeoxynucleotide (ASODN) mediated by jetPEI-RGD improved effect on colon carcinoma cell line caco-2. 目的：观察聚乙烯亚胺(PEI)-整合素蛋白的配体(Arg-Gly-Asp, RGD)介导的bcl-2反义核酸(antisense oligodeoxynucleotide, ASODN)对结肠癌细胞caco-2的作用效果。
Conclusion ATP7B antisense oligodeoxynucleotide increased the sensitivity of cisplatin in ovarian cancer cell line SKOV3ipl. 结论 ATP7B基因反义寡核苷酸能增加SKOV3ip1细胞对顺铂的敏感性。
Objectives: To study the inhibitory effects of mouse telomerase RNA (mTR) antisense oligodeoxynucleotide(ASODN) on telomerase activity in rat spermatogonia. 目的 :探讨小鼠端粒酶RNA(mTR)基因的反义寡核苷酸 (ASODN)对体外培养的大鼠A型精原细胞端粒酶活性及其mTR亚基表达的影响。
Objective: To explore the effect of liposome transfection antisense phosphorothioate oligodeoxynucleotide directed against the coding region of the bcl-2 messenger RNA and the translation site, and G3139 on apoptosis in Raji cells. 目的:通过脂质体分别转染bcl-2两个靶点的反义寡核苷酸(ASODN1、ASODN2)及G3139,观察bcl-2反义寡核苷酸对淋巴瘤细胞株Raji凋亡的影响。
Objective:To explore the effect of antisense phosphorothioate oligodeoxynucleotide (ASODN) directed against the 5 untranslated region, the translation site and the coding region of the bcl 2 messenger RNA on apoptosis in K562 cells. 目的 :探讨bcl 2不同靶点的反义寡核苷酸 (ASODN)对白血病细胞株K5 6 2细胞凋亡的影响。
I focus on the role of antisense RNAs in plasmid replication. 本文着重叙述反义RNA在质粒复制中的作用及其进展。
Poorly differentiated nasopharyngeal carcinoma (SUNE 1) cell line was cultured with Epstein Barr virus (EBV) BHRF1, DNase, thymidine kinase (TK) and nuclear antigen 1 (EBNA1) antisense oligodeoxynucleotide (ASO) for 48 h respectively. 用不同浓度的EB病毒 4种 (BHRF1、DNA酶、核抗原 1及胸苷激酶 )反义寡核苷酸片段 ,(antisenseoligodeoxynucleotide ,ASO)分别与含 10%25胎牛血清或不含胎牛血清的人低分化鼻咽癌细胞株 (SUNE 1)共同培养 4 8h。
Applying antisense oligonucleotide to block the expression of CLC-3 mRNA. 应用反义核酸的阻断CLC一3 mRNA在细胞中的表达。
Objective:In order to explore the effects of CD40 antisense RNA on Balm cell. 目的：探讨CD40反义RNA对Balm细胞的影响。
Meanwhile, the application of antisense gene technology is prospected. 并对反义基因技术的应用进行了展望。
Cyclin D3 antisense oligodeoxynucleotides were used in in vitro culture study. 用反义技术和流式细胞术研究周期蛋白D3在白血病细胞增殖中的作用。
However, the molecular mechanism of this antisense regulation remains unknown. 然而这种反义调节的分子机理还不甚明了。
Objective To study the immunotherapy role of CpG ODN(CpG oligodeoxynucleotide)on tumor. 目的探讨合成含CpG基序的寡核苷酸(CpG ODN)在肿瘤免疫治疗中的研究。
Methods Phosphorothioate oligodeoxynucleotide containing 2 CpG motifs was synthesized as the adjuvant of HBsAg. 方法以血源HBsAg为接种疫苗,人工合成含CpG基序的硫代磷酸寡脱氧核苷酸作为佐剂,以BALB/e小鼠作为实验动物进行免疫接种;