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- In this experiment PST lipsome was produced by freeze thawing method. 采用冰冻熔融法制备脂质体,包封率进行测定可达29%25。
- The CMO ORF was cloned and the plant expression vector pBI121-CMO was constructed. The resultant plasmids pBI121-CMO was transferred into Agrobacterium tumefaciens (GV3101) by the liquid nitrogen freezing thaw method. 克隆了CMO基因的编码区,构建了其植物表达载体pBI121-CMO,冻融法将质粒导入根癌农杆菌(Agrobacterium tumefaciens)GV3101,获得工程菌GV3101-pBI121-CMO。
- Methods The tumor antigen peptides from 4T1 breast cancer cells were acquired by freeze thawing method and HSP70-4T1 peptides complex was reconstructed in vitro. 方法冻融法获取BALB/C小鼠4T1乳腺癌细胞抗原肽,体外重组HSP70-4T1抗原肽复合物。
- freeze - thaw method 冻融法
- Leave the chicken out overnight to thaw it out. 把这只鸡在外放一晚上就解冻了。
- Come up to the fire, and you will thaw out. 到火炉边来，你就会觉得暖和。
- The method seems good but it needs to be tried out. 这个方法似乎不错，但需要试验一下。
- In this paper, the thawing methods and their characteristics were introduced. 这里对各种解冻方法及其特点进行了分析。
- A cup of tea helped to thaw out our guest. 一杯茶有助于我们的客人变得不拘束。
- After a good dinner he began to thaw. 吃过一顿美餐后，他开始变得随和了。
- The spring thaw often caused flooding. 春天解冻常常引起洪水泛滥。
- The government has introduced a wage freeze. 政府已宣布要冻结工资。
- Methods:The tests about the three key techniques:cryoprotective medium,freezing and thawing methods of Capella?s method were comparatively studied. 方法 :对经典的 Capella四步冻贮法的冷冻保护液、降温冷冻速率和复温方法 3项关键技术做了全面简化和改进。
- The sudden thaw means that spring is here. 一朝解冻，便是春天来到。
- Some fruits don't freeze well at all. 有些水果根本就不能冷藏。
- The cryopreservation and thawing method of porcine oocytes were reviewed.The effect and influence factors of preservation, choosing protection agents and so on were also discussed. 摘要综述了猪卵母细胞的冷冻保存方法和解冻方法，同时对保存效果、影响保存的因素、保护剂的选择等进行探讨。
- Shall I put some anti- freeze in the radiator? 要不要在散热器里注入一些防冻剂？
- Freezing thawing durability of both ordinarily and air entrained concrete exposed to Xinjiang, Qinghai, Inner Mongolia and Xizang salt lakes were determined by the accelerated freezing thawing method. 用快冻法研究了普通混凝土与引气混凝土在水和西藏、内蒙古、新疆和青海盐湖卤水中的抗冻性。
- She won't freeze you out if you call on her. 你如果去拜访她,她不会把你拒之门外的。
- The children's cheerful talk will help to thaw out guests. 孩子们欢快的谈话使客人们变得不那么拘束。
