Methods The tumor antigen peptides from 4T1 breast cancer cells were acquired by freeze thawing method and HSP70-4T1 peptides complex was reconstructed in vitro.

• 方法冻融法获取BALB/C小鼠4T1乳腺癌细胞抗原肽,体外重组HSP70-4T1抗原肽复合物。