The CMO ORF was cloned and the plant expression vector pBI121-CMO was constructed. The resultant plasmids pBI121-CMO was transferred into Agrobacterium tumefaciens (GV3101) by the liquid nitrogen freezing thaw method.

• 克隆了CMO基因的编码区,构建了其植物表达载体pBI121-CMO,冻融法将质粒导入根癌农杆菌(Agrobacterium tumefaciens)GV3101,获得工程菌GV3101-pBI121-CMO。