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- Conclusion The SNP fluorescent-multiplex system based on the fragment length discrepant allele specific PCR strategy is simple and economical, and is of a high application value in forensic medicine. 结论采用荧光标记复合扩增毛细管电泳法进行SNP分型，方法简单实用，在法医学个人识别领域具有较高的应用。
- We genotyped some main Chinese sweet cherry cultivars for the first time using the alleles specific PCR, and two new S genotype (S1S6, S4S9) were found, which made the listing of S-allele assignment more completed. 对57个国内外的甜樱桃品种进行了自交不亲和(SI)基因型的鉴定，首次鉴定了我国主栽的一些甜樱桃品种的S基因型，并发现了两个新的S基因型S1S6、S4S9，将自交不亲和的组群扩展到21个。
- Keywords Plasmodium vivax;circumsporozoite protein（CSP）;nest allele specific PCR;different size DNA band; 间日疟原虫;环子孢子蛋白基因;套式等位特异PCR;多样性DNA片段;
- Keywords Forensic biological evidence;Y chromosome;Biallelic marker;Tragment length discrepant allele specific PCR;Haplogroup; 法医物证学;Y染色体;双等位基因标记;片段长度差异等位基因特异性PCR;单体群;
- allele specific PCR 等位基因特异性多聚酶链反应
- allele specific PCR(AS-PCR) 等位特异性PCR, 等位基因特异性扩增技术
- Methods:Polymerase chain reaction in combination with dot blot hybridization of allele specific oligonucleotide(PCR/ASO) probes was used. 方法：采用聚合酶链反应结合等位基因特异的寡核苷酸探针杂交（PCR/ASO）技术。
- The methylation specific PCR (MSP) and bisulfite DNA sequencing were performed to examine the methylation status of SLIT2 gene promoter. 亚硫酸氢钠处理DNA,用MSP和测序法检测SLIT2启动子甲基化状态。
- The sensitive and specific PCR method for detection E. granulosus from dog faeces was preliminarily developed. 初步建立了灵敏、特异的检测家犬粪便中细粒棘球绦虫的PCR方法。
- Method Several pairs of specific PCR primers were designe d according to the conserved region of HDV genome. 方法针对HDV基因保守区域设计多对PCR引物。用芯片点样仪将PCR产物点到玻片上制成基因芯片。
- Conclusion The sensitive and specific PCR method for detection E. granulosus from dog faeces was preliminarily developed. 结论初步建立了灵敏、特异的检测家犬粪便中细粒棘球绦虫的PCR方法。
- Methods The methylation status of CpG island of AR gene promoter in 3 leukemic cell lines and 15 leukemic bone marrow cells was detected by methylation specific PCR(MSP). 方法应用甲基化特异性PCR技术(methylation specific PCR,MSP)检测3种白血病细胞株及15例患者骨髓标本雄激素受体基因甲基化状态。
- Methods The specific PCR primers were designed and synthesized to amplify the DNA fragments enconding preS1/S2/S antigen gene from the plasmid pHBV adr by PCR method. 方法设计合成2对寡核苷酸引物,以adr亚型HBV质粒pHBV DNA为模板,采用PCR法分别扩增HBV大分子表面蛋白基因(preS1/S2/S基因)片段;
- The established specific PCR assay should provide a practical and rapid method for the identification and differentiation of Eimeria species in mixed infections. 用本项研究所建立的特异PCR方法对这些混合感染的球虫种类进行鉴定具有一定的实用价值。
- allele specific amplification(ASA) 等位基因特异性扩增
- allele specific oligonucleotide(ASO) 等位基因特异性寡核苷酸, 等位基因特异的
- Using methylation specific PCR (MSP) to detect the CpG island methylation changes of PRA & PRB promoters in the leukemia cell lines before and after 5-Aza CdR treatments and the MG88 transfection. 利用MSP检测白血病细胞株在5-Aza CdR处理前后、MG88的转染前后PRA、PRB启动子CpG岛甲基化状态。
- allele specific oligonucleotide hybridization 等位基因特异寡核苷酸杂交
- Allele specific polymerase chain reaction 等位基因特异性聚合酶链反应
- Single-tube bi-directional allele specific amplification 单管双向等位基因专一性扩增
