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The characteristics of labelled MSCs differentiating into osteoblasts in vitro were identified by ALP stain,Von Kossa stain and bone morphogenetic protein3(BMP3) expression. 选用碱性磷酸酶染色、Von Kossa染色及骨形成蛋白3(BMP3)基因表达分析等技术,观察标记后MSCs体外分化成骨细胞的特性。
BEST OF ALL, KENTON'S METHOD IS EASY - REALLY EASY! 最重要的是，肯的方法很容易-真的容易！
Cells treated with 25-hydroxycholesterol appeared many cell nodules with positive von Kossa staining.Both insoluble calcium and medium osteocalcin increased significantly. 由此可见体外培养的主动脉中膜细胞有两种亚型，一种为平滑肌细胞的表型；
Cells were then transfected with AAV-hBMP4 and AAV-EGFP respectively. The ossification of cells was evaluated by morphological observation,alkali phosphatase stain,Von Kossa stain and alkali phosphatase assay. AAV-hBMP4与对照病毒AAV-EGFP分别转染细胞,观察细胞形态,行碱性磷酸酶(ALP)染色、Von Kossa染色及ALP含量测定,观察成骨活性。
Treatment＇ s method：to wear the glasses after ake the pleoptics,to operate. 结果诊治年龄越小、弱视的程度越轻、疗程越长的患者预后越好。
Methods The suggestibility was eveluated acording to the Tang s method the personality was evaluated with EPQ. 方法以医护人员为被测对象,完全依照唐安平等人的暗示性测查方法,测查其暗示性并分别给予艾森克个性问卷(EPQ)测查。
But he has annoyed some mainstream green groups by asking them to denounce the ELF’s and ALF’s methods. 这两个团体拒绝被称作“恐怖分子”，而且宣称他们会采取一切措施来预防对动物（也包括人类）的伤害。
In the conclusion part, the author summariesthe research of Data Mining 's method. 在第四章结论部分,对本文在数据挖掘方法上的研究工作进行了总结。
The new estimator gives threshold than Kay?s method while retaining the unbiased property and estimation range. 新的估计方法的方差具有更低的输入信噪比阈值,而保持了Kay的方法的无偏性和频率估计范围。
Numerical results of the model problem show that the method presented in this paper is betterthan Evan,s method. 模型问题的数值结果表明，本方法比Evans的AGE方法好。
In the example, B 's method takes precedence over the first extension method, and C 's method takes precedence over both extension methods. 例子中，B的方法优先于第一个扩展方法，C的方法优先于两个扩展方法。
METHODS: Nephrotoxic serum nephritis model of rat was cre ated by Clarke s method, and changes in ultrastructure of kidney were examined. 方法:采用大鼠肾毒血清性肾炎模型,常规电镜制片、染色,观察肾组织超微结构的变化。
Methods:The tests about the three key techniques:cryoprotective medium,freezing and thawing methods of Capella?s method were comparatively studied. 方法 :对经典的 Capella四步冻贮法的冷冻保护液、降温冷冻速率和复温方法 3项关键技术做了全面简化和改进。
Von Kossa's methods [医] 冯科萨氏法(检硝酸银法)
Conductive polymer poly (p-phenylene vinylene) (PPV) and precursor of PPV were prepared via Wessling’s method. 采用Wessling的前驱聚合物法获得导电聚合物PPV前驱体。
For example, a member identifier declared in a class type might be redeclared in the block of one of the class’s methods. 例如，类类型中声明的成员标识符可能在类的方法的某个块中被再声明。
Deep water driller s method is a suitable well killing method for deep water well control. 摘要深水司钻法是一种适应于深水井控的压井方法。
In the example, B's method takes precedence over the first extension method, and C's method takes precedence over both extension methods. 例子中，B的方法优先于第一个扩展方法，C的方法优先于两个扩展方法。
Conclusion:The device of Millard s method conforms to the requirement of reconstructing the lip on the physiolog... 结论:旋转推进法的设计更符合对唇裂修复的生理性原则,而下三角瓣法设计简单,并认为这两种方法能长期并存却不能相互取代。
Methods RBC-C3b rosette test, RBC-IC rosette test and direct tumor erythrocyte rosette test by Guo-feng s method were used. 方法采用郭峰法红细胞C_(3b)受体花环、免疫复合物花环实验和直向肿瘤红细胞花环试验测定红细胞免疫粘附功能。