Cells were then transfected with AAV-hBMP4 and AAV-EGFP respectively. The ossification of cells was evaluated by morphological observation,alkali phosphatase stain,Von Kossa stain and alkali phosphatase assay.

• AAV-hBMP4与对照病毒AAV-EGFP分别转染细胞,观察细胞形态,行碱性磷酸酶(ALP)染色、Von Kossa染色及ALP含量测定,观察成骨活性。