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- Tth DNA polymerase Tth DNA 聚合酶
- Tth DNA polymerase Tth DNA聚合酶[来自嗜热细菌Thermus thermophilus的耐热DNA聚合酶, 有逆转录活性]
- FastStart Taq DNA Polymerase, December ed. 酸外切酶活性( Roche Package Insert.
- Using chromosome DNA of Lactobacillus casei 34103 as template, thy A (Thymidylate synthase) gene was amplified by PCR with pfu DNA polymerase. 以干酷乳杆菌L.;casei34103染色体DNA为模板,利用PCR技术扩增胸苷酸合成酶(Thymidy-1atesynthase,fhyA)基因,回收纯化。
- Telomerase is RNA dependent DNA polymerase, acts to synthesize DNA repeat fragment of telomeres , which is associated with immortalization of cells. 端粒酶是一种RNA依赖的DNA多聚酶,可以合成端粒DNA的重复片段与细胞永生化密切相关。
- First, this term is often used to describe both the viral subtype (A,B,C,D, etc) as well as drug resistance mutations in the HBV DNA polymerase gene. 首先,这个术语经常被用来描述病毒亚型(A,B,C,D等)以及HBV DNA多聚酶基因中出现的耐药性突变。
- Full-length plasmid DNA was amplified with the above primers with a high-fidelity thermostable DNA polymerase capable of long-rang amplification. 用能扩增长片段的高保真耐热 DNA聚合酶扩增全长的质粒DNA,直接转化大肠杆菌。
- Phylogeny within DNA polymerase X family requires a re examination as the expansion of family members,particularly considering the enter of two entomopoxvirus(EPV) members. 随着DNA聚合酶X家族成员数量的增加 ,特别是两个昆虫痘病毒 (entomopoxvirus ,EPV)成员的加入 ,家族内部的系统发育关系需要重新检查。
- Further studies showed that DNA replication fidelity is decreased and there is an alteration of DNA polymerase spectrum when mammalian cells are attacked by MNNG. 因此,我们推测,哺乳细胞中低保真度的跨损伤DNA聚合酶可能参与了MNNG诱导的哺乳细胞非定标突变。
- A system used for the determination of fidelity of DNA polymerase in PCR was developen in E.coli and was used to determine the fidelity of FD DNA polymerase in PCR amplication. 摘要 在大肠杆菌中建立了一套用于测定 DNA聚合酶在PCR过程中复制精确性的系统,并测定了耐热FD DNA聚合酶在PCR扩增过程中的复制精确性。
- In molecular biology, processivity is a measure of the average number of nucleotides added by a DNA polymerase enzyme per association/disassociation with the template. 应该说PCR其他的技术层次都已经很完善了,唯一的瓶颈是DNA聚合酶的持续合成能力(processivity)。
- Lamivudine, and most nucleoside analogues, acts by suppression of viral replication through competitive inhibition of reverse transcriptase and DNA polymerase. 拉米还有大多数的核苷类似物,通过竞争性地阻断翻转录酶和DNA聚合酶来抑制病毒复制。
- More specifically, amino acid substitutions occur in the tyrosine-methionine-aspartate- aspartate (YMDD) motif of the DNA polymerase of the virus, which renders the drug much less effective. 更具体的说,病毒DNA聚合酶中的酪氨酸-蛋氨酸-天门冬氨酸-天门冬氨酸(YMDD)基序中发生氨基酸取代,导致药物失效。
- A gene encoding Trichosanth in (TCS)was amplified by means of DNA polymerase chain reaction(PCR) and cloned from the genomic DNA of Trichosanthes kirilowii. Its DNA sequence has been determined. 本文应用DNA多聚酶链式反应(PCR)技术,从栝楼基因组DNA中扩增并克隆了天花粉蛋白(TCS)基因。
- Most mammalian cells contain multiple species of DNA dependent DNA polymerases. 大部分哺乳动物细胞均含有多种依赖DNA的DNA聚合酶。
- In Q group the gene expressions of DNA polymerase beta, EGFR and c-myc detected by RNA dot blot array were 6.5 folds, 7 folds and 2.2 folds down-regulated respectively, and the wtp53 was 2.5 folds up. Q组的DNAPolp,EGFR及e一mye的RNA斑点TLe扫描数值分别比对照组下调了6.;5倍,7倍,2
- Factors which affect the ISSR analysis of Abies beshanzuensis,such as annealing temperature and concentrations of template DNA,Taq DNA polymerase,Mg~2+ and dNTP,etc. were examined. 以百山祖冷杉DNA为材料;分析了DNA浓度、Mg2+浓度、dNTP浓度、Taq酶的含量以及退火温度对ISSRPCR扩增结果的影响;经过优化实验建立了百山祖冷杉ISSRPCR最佳反应体系.
- The results of reverse spot hybridization for both duck hepatitis B virus DNA (DHBV DNA) and special DNA polymerase were compared with those by dot blot for DHBV DNA and electromicroscopy for DHBV particles. 本文将逆向斑点杂交同时检测鸭乙型肝炎病毒DNA(DHBV DNA)及其特异DNA多聚酶(DNAp)的结果,与斑点杂交检测DHBV DNA和电镜检测DHBV颗粒的结果作了比较。
- METHODS The contents of ET-1 in lung of asthmatic guinea-pigs were measured with radioimmunoassay, and the expressions of ET-1, ETA, ETB and ECEmRNA with reverse transcription DNA polymerase chain reaction (RT-PCR). 方法 放免法测定肺组织ET-1,逆转录多聚酶链式反应(RT-PCR)方法检测ET-1、ETA、ETB和ECE的mRNA表达量。
- After the second strand cDNA replacement synthesized, the uneven termini of the double- stranded cDNA were filled in with Puf DNA polymerase and EcoR I adapter were ligated to the blunt ends. cDNA第二链合成后用Pfu DNA聚合酶补平肠口I位点,再与EcoR AdaPters连接,经乃。