Factors which affect the ISSR analysis of Abies beshanzuensis,such as annealing temperature and concentrations of template DNA,Taq DNA polymerase,Mg~2+ and dNTP,etc. were examined.

• 以百山祖冷杉DNA为材料；分析了DNA浓度、Mg2+浓度、dNTP浓度、Taq酶的含量以及退火温度对ISSRPCR扩增结果的影响；经过优化实验建立了百山祖冷杉ISSRPCR最佳反应体系.