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- 采用Trizol试剂对病毒进行了核酸提取,电泳结果发现有4条核酸条带,分别为3.0kb、1.3kb、0.8kb、0.75kb,对RNA核酸酶、S1核酸酶敏感,对DNA酶抵抗,为单链RNA病毒。 The nucleic acid of virus was extracted by using the Trizol reagents and analyzed with 1%25 agarose electrophorosis. It shows the nucleic acid has four bands sized 3.0, 1.3, 0.8 and 0.75 kb respectively, and is sensitive to RNase and S1 nuclerase and resistant to DNase, indicating these bands as single?strand RNA.
- HIV-1逆转录酶(reverse transcriptase RT)在病毒生命周期中起重要作用。 病毒通过逆转录酶将单链RNA逆转录为双链DNA,随后病毒DNA迅速整合到宿主DNA中,从而完成转录、翻译等整个生命周期。 The reverse transcriptase (RT) plays a crucial role for the replication of HIV type-1 (HIV-1) in the virus life cycle, and is responsible for the convertion of the single-stranded RNA viral genome into double-stranded DNA that subsequently integrates into the host DNA.
- 双链RNA表达 dsRNA expression
- 短链RNA干扰 short interfering RNA
- 单链RNA single-stranded RNA
- 双链RNA分解酶 dsRNA dependent ribonuclease
- 小的干涉双链RNA siRNA
- 单链线 single catenary
- 单链噬菌体 single stranded phage
- 烟草花叶病毒的单份基因组是一个6395个核甙的(+)链RNA分子。 The monopartite genome of tobacco mosaic virus is a plus-strand RNA molecule of 6395 nucleotides.
- 单链DNA single-stranded DNA; ssDNA
- 单链环 single-stranded loop
- 单链泡 single-strand bubble
- 背景和目的:博尔纳病病毒(Borna disease virus, BDV)是一种有套膜的非分节段的单分子负链RNA病毒。 Background and Objective:Borna disease virus (BDV) is an enveloped, nonsegmented, mononegative RNA virus.
- 单链Fab single chain Fab
- 单链表 Single linked list
- 单链传动 single-chain drive
- 单链悬挂 single catenary suspension
- 单链 DNA single-stranded DNA; ssDNA
- 单链 RNA single stranded RNA; ssRNA