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VMAT2 transgenic CHO cells can resist the toxicity of dopamine. 转染VMAT2的CHO细胞对DA引起的毒性有显著的抵抗作用。
transgenic CHO cell 转基因CHO细胞
VMAT2 has protective effect on transgenic CHO by transporting MPP+ to vesicles. 此保护机制是由转基因细胞中VMAT_2引起的,VMAT_2在转基因的非神经细胞系(CHO细胞系)中也能将MPP~+转运至囊泡内,从而保护细胞,同时也提示PC12细胞内具有抗毒性作用的成分。
Aim To construct a CHO cell expression vector carrying coamplification gene. 目的构建携带共扩增基因的CHO细胞表达载体。
Conclusion A model for the construction of engineered CHO cell line producing recombinant protein with CHO/DHFR system is established. 结论应用CHO/DHFR表达系统，为构建CHO细胞的工程细胞株建立了良好的模型。
As detected by immunocytochemical assay, all of these four plasmids could express core protein after transfected into CHO cell line. 免疫细胞化学实验表明，这四个质粒转染CHO细胞后都能在细胞内表达核心蛋白。
Results After one cycle of amplification by methotrexate(Mtx), the CHO cell colonies stably expressed green fluorescent protein. 结果经脂质体法转染CHO细胞，以氨甲喋呤为选择标记，经过一轮扩增后，获得表达绿色荧光蛋白的CHO细胞株。
Using porous microcarrier Cytopore, a recombinant CHO cell line C2 producing have been successfully cultivated in 2.2 celligen perfusion culture system. 在2.;2L celligen灌注培养系统中采用Cytopore多孔微载体培养产重组人促红细胞生成素（rHEPO）的细胞C2。
Human brest cancer cell MCF-7 was transfected with pcDNA3.1(-)/VEGF-C by using lipofectamine transfection reagent and screened with antibiotic G418. The expression of VEGF-C protein in CHO cell and supernatant was identified with Western-blotting. 最后用Western -blotting法检测细胞培养上清以及裂解细胞中的VEGF -C蛋白。
In conclusion, the model system of co-expressing MOR and I1R in CHO cells is generated for the first time. 综上所述，本文在国内外首次建立了共同稳定表达MOR和I1R的哺乳动物细胞表达系统；
In order to get recombinant BPI, the gene was cloned into an expressing plasmid and expressed in CHO cells. 构建真核表达质粒 ,在CHO细胞中实现BPI基因的稳定表达。
The serum-containing medium for culture of CHO cells can be completely substitutedby serum-free media. 无血清培养基可以完全取代含血清培养基用于培养CHO细胞。
The CHO cells that could steadily express at high level the CTLA-4/Ig fusion protein was selected for cloning. 筛选出了能高效稳定表达CTLA-4/Ig融合蛋白的CHO细胞;
Method ANP cDNA transfected CHO cells were encapsulated in PCL tubes and levels of ANP secreted were detected. 方法将人ANP基因(cDNA)转染入CHO细胞,然后将细胞包被在聚己内酯(PCL)管内形成微囊,并检测转基因CHO细胞长时间存活情况和分泌的ANP。
Results The bands of MOR and OTR were seen in the cotransfected CHO cells and rat brain tissue via IP test. 结果IP方法检测显示，在MOR和OTR质粒共转染CHO细胞及大鼠脑组织中可见MOR和OTR蛋白条带。
Conclusion The heterodimers of MOR and OTR exist in the cotransfected CHO cells and brain tissues. 结论在MOR和OTR质粒共转染CHO细胞及大鼠脑组织中存在异源性MOR和OTR二聚体。
CONCLUSION: Sodium pentachlorophenol could induce DNA damage and chromosome aberration of CHO cells. 结论：五氯酚钠能诱发CHO细胞DNA损伤和染色体畸变。
B7 transfected CHO cells were used as artificial antigen presentation cells (APCs) in MLR to exclude the effects of other costimulatory molecules. 为了排除其它粘附分子的作用 ,应用联合转染DR7或 (和 )B7分子基因的CHO细胞系作为人工抗原递呈细胞 (APC)介导混合淋巴细胞反应 (MLR)。
Aftertransected with the expression vector , CHO cells can express the fusion protein stably , which was con-firmed by Western blot and ELISA. Western blot及ELISA法检测到细胞培养上清中有融合蛋白的表达。
The retention capability of CTX is demonstrated to be heparan sulfate-dependent by retention test on immobilized CHO cells. 心脏毒蛋白在细胞上的留滞能力跟细胞上的肝素硫酸有关。