Human brest cancer cell MCF-7 was transfected with pcDNA3.1(-)/VEGF-C by using lipofectamine transfection reagent and screened with antibiotic G418. The expression of VEGF-C protein in CHO cell and supernatant was identified with Western-blotting.

• 最后用Western -blotting法检测细胞培养上清以及裂解细胞中的VEGF -C蛋白。