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- The methylation specific PCR (MSP) and bisulfite DNA sequencing were performed to examine the methylation status of SLIT2 gene promoter. 亚硫酸氢钠处理DNA,用MSP和测序法检测SLIT2启动子甲基化状态。
- The sensitive and specific PCR method for detection E. granulosus from dog faeces was preliminarily developed. 初步建立了灵敏、特异的检测家犬粪便中细粒棘球绦虫的PCR方法。
- Method Several pairs of specific PCR primers were designe d according to the conserved region of HDV genome. 方法针对HDV基因保守区域设计多对PCR引物。用芯片点样仪将PCR产物点到玻片上制成基因芯片。
- Conclusion The sensitive and specific PCR method for detection E. granulosus from dog faeces was preliminarily developed. 结论初步建立了灵敏、特异的检测家犬粪便中细粒棘球绦虫的PCR方法。
- Methods The methylation status of CpG island of AR gene promoter in 3 leukemic cell lines and 15 leukemic bone marrow cells was detected by methylation specific PCR(MSP). 方法应用甲基化特异性PCR技术(methylation specific PCR,MSP)检测3种白血病细胞株及15例患者骨髓标本雄激素受体基因甲基化状态。
- Methods The specific PCR primers were designed and synthesized to amplify the DNA fragments enconding preS1/S2/S antigen gene from the plasmid pHBV adr by PCR method. 方法设计合成2对寡核苷酸引物,以adr亚型HBV质粒pHBV DNA为模板,采用PCR法分别扩增HBV大分子表面蛋白基因(preS1/S2/S基因)片段;
- The established specific PCR assay should provide a practical and rapid method for the identification and differentiation of Eimeria species in mixed infections. 用本项研究所建立的特异PCR方法对这些混合感染的球虫种类进行鉴定具有一定的实用价值。
- Using methylation specific PCR (MSP) to detect the CpG island methylation changes of PRA & PRB promoters in the leukemia cell lines before and after 5-Aza CdR treatments and the MG88 transfection. 利用MSP检测白血病细胞株在5-Aza CdR处理前后、MG88的转染前后PRA、PRB启动子CpG岛甲基化状态。
- We genotyped some main Chinese sweet cherry cultivars for the first time using the alleles specific PCR, and two new S genotype (S1S6, S4S9) were found, which made the listing of S-allele assignment more completed. 对57个国内外的甜樱桃品种进行了自交不亲和(SI)基因型的鉴定,首次鉴定了我国主栽的一些甜樱桃品种的S基因型,并发现了两个新的S基因型S1S6、S4S9,将自交不亲和的组群扩展到21个。
- Conclusion The SNP fluorescent-multiplex system based on the fragment length discrepant allele specific PCR strategy is simple and economical, and is of a high application value in forensic medicine. 结论采用荧光标记复合扩增毛细管电泳法进行SNP分型,方法简单实用,在法医学个人识别领域具有较高的应用。
- Use Species - specific PCR Technology Identify Fusarium moniliform 应用PCR技术检测串珠镰刀菌
- Education should not be restricted to any one specific age group. 教育不应限制在任何特定的年龄组上。
- Keywords Bungarus multicinctus;high specific PCR;identification; 金钱白花蛇;高特异性鉴别;PCR;
- Keywords Lung cancer;Epithelial Cadherin;Methylation specific PCR; 肺癌;上皮钙粘蛋白;甲基化特异性PCR;
- He gave us very specific instructions. 他给我们做了非常明确的指示。
- There is a specific tool for each job. 各工种配备特定的工具。
- But that all depend on the specific circumstances. 不过,那要看具体情况而定。
- He held fire on specific foreign policy question. 他对具体的外交政策暂不表态。
- There is no specific remedy for the malady. 没有医治这种病的特效药。
- Quinine is a specific for malaria. 奎宁是治疗疟疾的特效药。