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- The construction of recombinant vector containing Smac/ DIABLO gene. 构建含有Smac/DIABLO基因片断的重组质粒pcDNA3.;1 /HisA-Smac/DIABLO。
- The obtained recombinant vector, named pGEM mimic, can be used as a common competitor for either b3a2 or b2a2 type of bcr abl cDNA. 较b2a2型bcr－ablcDNA相应的201bp扩增片段长39bp，使之适于作为b3a2和b2a2型两类bcr－ablmRNA定量PCR的通用内标物。
- Recombinant vector of pUCm-T/mIA-2i-mIgG Fc was prepared by gene combination and confirmed by PCR and dual-site endonuclease. 获得重组克隆质粒pUCm-T/mIA-2i-mIgGFc,经聚合酶链反应和双位点酶切鉴定重组成功。
- Objective To construct a recombinant vector which expresses active phenylalanine-amonia-lyase(PAL) in Lactococcus lactis (L. 的构建能表达活性苯丙氨酸脱氨酶(PAL)的重组质粒，使其在乳酸乳球菌(L.
- Objectives To construct a recombinant vector containing fragments of emm 1 genes and emm 3 gene that are the type specific epitopes of streptococcus pyogenes. 目的构建含A组链球菌M蛋白基因(emm基因)1型和3型特异性抗原决定簇基因的重组质粒。
- Hep2 cells were transfected by the recombinant vector and apoptosis of the transfected cells was evaluated by trypan-blue exclusion and the agarosegel electrophoresis of DNA. 将该重组载体经阳离子脂质体转染入人喉癌细胞株Hep2中, 以台盼蓝拒染法和基因组DNA琼脂糖凝胶电泳, 观察转染的Hep2细胞的凋亡。
- The prokaryotic expression plasmid pGEX-IL-24 was constructed,GST-IL-24 molecular mass was approximately 50 000.Conclusion GST-IL-24 fusion protein was expressed in E. coli BL21(DE3) which was transformed from the recombinant vector of IL-24. 结论IL-24的重组载体在大肠杆菌BL21(DE3)可以表达GST-IL-24融合蛋白。
- We have made up an eukaryotic (Pichia pastoris) expressive vector (pPic9k-DCN). After the linear recombinant vector was introduced into HIS~-/GS115 cells,we have selected positice clones against G418(4mg/ml)and confirmed by PCR. 构建了真核表达载体pPic9k-DCN，并将酶切线性化的重组载体转入酵母菌HIS~-/GS115后，筛选出了抗高浓度(4mg/ml)G418的阳性克隆，并用PCR法进行了鉴定。
- Comparison between the two activities in COS-7 c ells transfected with the recombinant vector containing the prolidase gene and t he control cells showed parallel elevation with a constant ratio. 比较转染了带有氨酰基脯氨酸二肽酶基因的重组载体的COS 7细胞和对照组细胞中的两种酶活性 ,可以看到有平行的升高趋势及恒定的酶活性比值。
- Meanwhile, the feline IL-18 gene was subcloned into the prokaryotic expressing vector pET28a and recombinant vector was further transformed into Escherichia coli strain BL21(DE3) for expression under the induction of IPTG. 将目的基因片段进一步亚克隆到大肠杆菌(Escherichia coli)表达载体pET28a中构建了重组质粒pETIL-18,转化大肠杆菌BL21(DE3),并用IPTG诱导。
- Methods Antisense TRP-1 recombinant vector was transfected into melanocytes and malignant melanoma cell line,then these cells were observed under electron microscope and detected by flow cytometer. 方法 将TRP-1反义核酸真核表达载体转染至黑素细胞及恶性黑素瘤细胞，以透射电镜及流式细胞仪分别观察细胞的凋亡情况。
- Comparison between the two activities in COS-7 cells transfected with the recombinant vector containing the prolidase gene and the control cells showed parallel elevation with a constant ratio. 比较转染了带有氨酰基脯氨酸二肽酶基因的重组载体的COS-7细胞和对照组细胞中的两种酶活性，可以看到有平行的升高趋势及恒定的酶活性比值。
- The recombination vector that was named as pCAM-P was obtained based on the tests of PCR and enzyme digestion of the vector. 2. PCR检测、质粒酶切分析结果表明获得了Pto与pCAMBIA1300的重组子，命名为pCAM-P。
- Keywords NHE 1 gene;gastric neoplasm;antisense technology;recombinant vector; 1基因;胃肿瘤;反义技术;重组载体;
- Construction of Recombinant Vector for Expressing Leptospira Envelope Protein in BCG 钩端螺旋体外膜蛋白基因重组载体构建及在卡介菌中的表达
- Construction of a Transgenic Lycium barbarum L. Recombinant Vector Expressing HIV-1 Capsid Protein Viral Like Particles 转基因枸杞表达HIV-1壳体蛋白病毒样颗粒疫苗表达载体的构建
- Use menus to provide a pedagogic vector. 用菜单来提供一条学习的途径。
- That is transitory or semipersistent in its vector. 在它的介体中是属于中间的或半持久的。
- The vector product is said to be anticommutative. 可以说矢积是反交换的。
- Recombinant fragments;recombinant technology. 重组DNA碎片;重组DNA技术
