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- Samples were fixed for histology, and gene expression was assessed with a dual-dye 22 000 oligonucleotide microarray. 样品组织学固定,基因表达用双染的22000寡核苷酸微点阵评估。
- Oligonucleotide microarray technology is a powerful data-mining platform and has been widely applied in biosciences. 摘要寡核苷酸芯片技术是一种高通量发掘和采集生物信息的强大技术平合,目前已广泛应用于生物科学领域。
- On the basis of the first step of amplifying PCR, it has been hybridized with oligonucleotide microarray in order to analyze whether the amplified PCR product possess antimicrobial agent resistance or not. 摘要在第一步耐药性靶基因得到扩增的基础上,通过与芯片进行杂交来分析所扩增的PCR产物是否是已经发生了突变而能导致抗药性的基因。
- Gene expression profiles of SW1990, SW1990/R, ASPC and ASPC/R cells were screened using oligonucleotide microarray (Affymetrix HG U133 2.0plus) which contained approximately 38000 human genes. 应用基因芯片技术检测胰腺癌细胞株SW1990、ASPC及耐放射细胞亚株SW1990/R、ASPC/R的基因表达谱。 在47400个转录本中,通过Two way ANOVA分析得到与放射敏感性相关的统计学差异的基因共2392条,筛选亲本株与耐放射细胞亚株放射敏感性相关的差异在两倍以上的共同差异基因137条。
- To improve the performance of assays on the oligonucleotide microarray, the factors that influence the hybridization effects such as surface chemistry, probe size, spacer length, hybridization conditions etc were intensely studied and optimized. 为改善寡核苷酸芯片的分析性能,对影响芯片杂交结果的因素,如片基表面的化学处理、探针的长度、间隔臂的长度、杂交条件等,进行了深入的研究和优化。
- Keywords Pancreatic neoplasms;Oligonucleotide microarray;Gene expression; 胰腺肿癌;寡核苷酸芯片;基因表达;
- Preparation Optimization and Properties of the Aldehyde Microscopic Slides for Oligonucleotide Microarray Fabrication 用于寡核苷酸芯片制备载体的醛基载玻片的制备方法优化及性质研究
- Design and preparation of oligonucleotide microarray for vaccinia virus detection 痘苗病毒寡核苷酸检测芯片的设计及研制
- Construction and preliminary application of oligonucleotide microarray specialized for pancreatic adenocarcinoma 胰腺癌相关基因寡核苷酸芯片的制备和初步应用
- The impact of three fluorescence marker methods to oligonucleotide microarray hybridizesignal 三种荧光标记法对寡核苷酸芯片杂交信号的影响
- Development and Application of Oligonucleotide Microarray for Detection of SARS Coronavirus SARS冠状病毒检测寡核苷酸基因芯片的研制及应用
- Keywords physical restraint;adrenocorticotropic hormone;corticosterone;post-stress recovery;oligonucleotide microarray; 束缚应激;促肾上腺皮质激素;皮质酮;应激后恢复;基因芯片;
- The pilot study on development of lamivudine-resistance oligonucleotide microarray and its reliability in clinical application 检测拉米夫定耐药位点基因芯片的研制及其应用初探
- A Robust and Low-cost Substrate for Oligonucleotide Microarray Compared with CMT-GAPSTM Aminosilane Slide and Microscope Slide 三种寡核苷酸芯片片基的比较
- Development and application of oligonucleotide microarray for detecting rifampin-resistant Mycobacterium tuberculosis strains 检测耐利福平结核分枝杆菌寡核苷酸芯片的研制及应用
- Study on oligonucleotide microarray technique for detection and identification of Enterobacteriaceae foodborne infection bacteria 寡核苷酸芯片技术检测肠杆菌科食源性感染常见致病菌的研究
- Keywords component combination in Siwu Decoction (CCSWD);cell proliferation;hematopoiesis-related gene expression profile;oligonucleotide microarray; 四物汤化学成分组合;细胞增殖;造血相关基因表达谱;寡核苷酸芯片;
- Keywords oligonucleotide microarray;leukemia;recipient/sibling donor pair;differentially expressed gene;Hematopoietic stem cell transplantation; 寡聚核苷酸芯片;白血病;供受者对;差异表达基因;造血干细胞移植;
- Preparation of Oligonucleotide Microarray for Expression Detection of Virus-infection- associated Genes and Its Application in Screening Responsive Genes against HBV Infection 病毒感染相关基因微阵列的制备及其在HBV感染应答基因筛选中的应用
- oligonucleotide microarray 寡核苷酸微阵