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The results showed that melittin changed its orientation in the latter case. 从而说明跨膜电位造成了蜂毒肽在膜上的重新取向。
Objective: To study the effect of melittin on apoptsis and necrosis of osteosarcoma cell Tine U2OS in vitro. 目的：研究蜂毒素是否能够诱导骨肉瘤细胞U2OS凋亡与坏死。
Objective To study the antiangiogenic effects of Melittin on hepatocellular carcinoma and the relative mechanisms. 目的探讨蜂毒素抗肝癌血管生成的作用及其机理。
Melittin has many important functions, which have already attracted people more interested in studying it. 由于蜂毒肽具有很强的溶血作用，极大地限制了其在医药领域的应用。
The orientation of melittin binding to liposomes with or without transmembrane potential was investigated. 利用脂质体模型膜系统研究了跨膜电位对蜂毒肽在磷脂膜上取向的影响。
Objective:To isolate and purify melittin from bee venom and to establish an SDS PAGE and HPLC method for the determination of melittin content. 目的 :从蜜蜂毒中分离纯化蜂毒素 (melittin) ,建立以 SDS-聚丙烯酰胺凝胶电泳 (SDS- PAGE)及 HPL C进行蜂毒素定性定量分析的方法。
Results: (1) Mass spectrometry result of the melittin isolated and purified from bee venom showed that m/z was 2846 dalton. 结果表明:(1)蜂毒素样品电喷雾质谱在全扫描一级激发的条件下;分子离子峰为m/z 2846.;0;从而确定其分子量为2846 Dal;与蜂毒素的理论分子量相符;
PLA2 and its activator, melittin, as well as CCh significantly decreased the binding of [3H]N-methyl-scopolamine ([3H]NMS) to CCL137 cells. PLA_2及其激活剂蜂毒肽都能像CCh处理一样,降低CCL137细胞与[~3H]NMS的结合作用;
In order to keep the anti-rheumatic arthritis activity and reduce the hemolysis hypersuscepti-bility of melittin, a deletion peptide of melittin was synthesized. 为了保留蜂毒肽抗类风湿关节炎活性,降低溶血性和致敏性,设计合成蜂毒肽的片段,观察它对兔免疫性关节炎的治疗作用。
RESULTS: Recombinant adenoviral vectors containing melittin gene were successfully constructed, and it was melittin gene that could be transcripted proved by RT-PCR. 结果：携蜂毒素基因的重组腺病毒载体构建成功，RT-PCR实验表明蜂毒素基因可以得到转录。
By using the previously-established model of quantitative structure-activity relationships(QSAR) for melittin,six genes of melittin analogues were predicted and designed. 采用先前建立的蜂毒肽构效关系(QSAR)模型,预测设计了6条蜂毒肽类似物基因。
Adsorption of melittin into HBM destroys the insulating capability of HBM, causing membrane resistance to decrease and membrane capacitance to increase, thus accelerating the redox reaction for the negatively charged redox couple Fe(CN)_6 3-. 在双层膜体系上引入的蜂毒素可在膜表面上形成孔洞 ,破坏膜的绝缘性 ,降低膜电阻 ,增加膜电容 ,使带负电的探针Fe(CN) 6 3-的氧化还原反应速度加快
Methods:Melittin was isolated and purified from bee venom by gel filtration on Sephadex G 25,Sephadex G 50 and Sephadex G 75.Homogenicity and molecular weight of melittin were determined by SDS PAGE and HPLC. 方法 :用 Sephadex G- 2 5、Sephadex G- 5 0、Sephadex G- 75三步层析法从蜜蜂毒中分离纯化蜂毒素 ,以SDS- PAGE定性 ,以 HPL C进行含量测定。
The destination of this study is to design the melittin gene in order to reduce hemolysis and keep antibacterial activity to the most extent, and discuss the mechanism of hemolysis. 本研究的预期目的是通过改换蜂毒肽分子一级结构中的个别关键性氨基酸组份，在保留其抗菌功能的前提下，降低溶血活性，同时研究其溶血作用的生化机理。
The linear increase is attributed to the transient disruption upon melittin binding to the membrane, whereas 100% leakage is due to the pore formation induced by high concentration peptides. 推断是蜂毒蛋白吸附脂膜时造成瞬间的裂缝而使渗漏率线性增加;推断有100%25渗漏，是因蜂毒蛋白在膜上形成孔洞所致。
With a combination of high performance liquid chromatography and mass spectrometry,the membrane association state of melittin was detected by analyzing its trypsin-digested products. 采用高效液相色谱与质谱技术相结合方法,分析了蜂毒肽与磷脂膜作用后的胰蛋白酶酶解产物。
Keywords Bee Venom;Melittin;HPLC; 关键词蜂毒;多肽溶血毒;HPLC;
Keywords melittin;osteosarcoma;angiogenesis;nude mice; 蜂毒素;骨肉瘤;血管生成;裸小鼠;