Methods:Melittin was isolated and purified from bee venom by gel filtration on Sephadex G 25,Sephadex G 50 and Sephadex G 75.Homogenicity and molecular weight of melittin were determined by SDS PAGE and HPLC.

• 方法 :用 Sephadex G- 2 5、Sephadex G- 5 0、Sephadex G- 75三步层析法从蜜蜂毒中分离纯化蜂毒素 ,以SDS- PAGE定性 ,以 HPL C进行含量测定。