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- Conclusions It indicated that the method of in situ RT PCR is specific and sensitive,and will be useful for research on coxsackie virus B sustainable infection and related diseases. 而非感染的HeLa细胞则不着色。 结论原位RT-PCR法可特异地检测柯萨奇B病毒感染HeLa细胞中的病毒RNA,是一种特异和敏感的定位检测方法,可应用于研究该病毒的持续性感染及其与克山病等相关疾病的关系
- Experimental study on detection of viral RNA of Coxsackie virus B by means of in situ RT PCR 原位RT-PCR法检测柯萨奇B病毒RNA的实验研究
- in situ RT - PCR 原位RT-PCR
- Methods RT polymerase chain reaction(RT PCR), immunohistochemical methods and in situ hybridization technologies were used to detect the PCNA, L faabp mRNA level and located distribution at embryonic period and natal period. 方法利用免疫组织化学、原位杂交和反转录聚合酶链反应(RT-PCR)等技术,分别检测胚胎期E14d,E17d,E19d和幼鼠期P1d,P2d,P7d,P28d大鼠小肠上皮细胞增殖细胞核抗原(PCNA)、L-fabpmRN水平及其定位分布。
- Key around Mobile, the next key stop in situ fixed. 左右键移动,下键停在原地不动。
- Lobular carcinoma in situ is seen here. 图示小叶原位癌。
- Fluorescence in situ hybridization See FISH. 荧光原位杂交:参见FISH.
- RDP=rumen-degradable protein measured in situ. 经瘤胃消化袋测得之瘤胃可分解蛋白质。
- Biopolymer can be triggered in situ to form a gel. 对生物矿化作用的进一步研究有助于微生物调剖机理的完善和应用。
- The rocks were not in situ; they had been brought from elsewhere. 石块不是原来就在那里的,是从别处搬来的。
- Methods:Nine Chinese AIDS kidney autopsies were detected by PCR in situ . 方法:应用原位PCR方法检测9例国人艾滋病尸体解剖肾组织标本。
- In situ deposits are dark shale with horizontal bedding. 原地沉积为水平层理发育的暗色泥板岩。
- In Situ Hybridization on Vasa Gene of Silkworm Bombyx Mori. 家蚕vasa基因的原位杂交研究
- Fluorescence in situ hybridization (FISH) technique developed. 发明原位免疫荧光杂交(FISH)技术.
- The debate here is probably ADH or ductal carcinoma in situ (DCIS). 这里争论的焦点是ADH还是导管原位癌。
- Methods Immunocytochemistry and in situ hybridization. 方法免疫细胞化学和原位杂交技术。
- It is the first report for in situ cohybridization in plants. 用生物素标记对它们进行了原位单杂交和共杂交的比较定位。
- Expression of P53 oncogene was detected by in situ hybridization . P53基因异常表达采用原位杂交方法。
- Methods: Non-radiative prober is used in hybridization in situ. 方法非放射性探针原位杂交法。
- Laser in situ Keratomileusis Retreatment for Residual Myopia[J]. 引用该论文 沈政伟;尹禾;梅军;吴金桃;李丽;黄震晞.