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- MethodsThe optimized host cell,culture medium and induction time were deternined according to the growth and expression specificity of nrhTNF on test tube and flask shaker. Then fed batch culture was carried out on 5 L automatic fermentor. 方法通过探讨nrhTNF工程菌在试管和锥形摇瓶中的生长和表达规律 ,筛选并确定其最适宿主菌、最佳培养基及最佳诱导表达条件 ,然后在 5L自控发酵罐中进行分批补料培养。
- In fed batch culture,the activity of neutral protease was further increased to 17. 67 g/min L by controlling the feeding rates of glucose and other niedium components. 在控制补加葡萄糖和其它培养基成分的补料分批培养中，中性蛋白酶活性达17.;670g/mm·L。
- Study of Substrate Inhibition and Fed Batch Culture in The Fermentation of D-Ribose D-核糖发酵过程中底物抑制及补料的研究
- Keywords ALDC;fed batch culture;high density culture;engineering bacteria;extracellular enzyme; 乙酰乳酸脱羧酶;分批补料;高密度培养;基因工程菌;胞外酶;
- Keywords pyruvic acid;Torulopsis glabrata;glucose;initial glucose concentration;batch culture;fed batch culture; 丙酮酸;光滑球拟酵母;葡萄糖;初始葡萄糖质量浓度;分批培养;补料培养;
- Keywords Torulopsis glabrata;pyruvic acid;nutritional conditions;ratio of C:N;fed batch culture; 丙酮酸;光滑球拟酵母;营养条件;碳氮比;流加培养;
- fed batch culture 加料分批培养
- MethodsThe study was in flask and fermentation tank culture by batch and fed batch method. 方法通过摇瓶培养和分批及补料分批发酵培养进行研究。
- The optimum culture conditions of genetically engineered Pichia pastoris in shake flask cultivation and in fed batch fermentation were investigated respectively. 在摇瓶条件下对基因工程菌 Pichia pastoris的发酵条件进行了实验 ,并根据摇瓶发酵的优化结果进行了补料分批高密度发酵。
- Objective: To prepare recombinant granulysin(GNLY) by fed batch cultivation of E. coli BL21(DE3)pLysS in a FUS-50L bioreactor. 目的:在FUS-50L生物反应器中利用补料分批培养技术培养表达含重组质粒pET28a(+)-GNLY的大肠杆菌BL21(DE3)pLysS株,生产重组颗粒溶素(GNLY)。
- A new type of overflowing jetting photobioreactor was used for the indoor batch culture of Spirulina . 采用一种新型溢流喷射光生物反应器进行螺旋藻的室内分批培养试验。
- The effect of fed batch system with the measuring tank on the fermentation of a newly selected strain,Aspergillus niger wml-016,was studied in a 50 L automatic fermentor. 选择计量罐式连续流加补料系统,采用新选育的黑曲霉(Aspergillus niger)Wml-016菌株在50 L自动发酵罐上研究间歇补料对发酵的影响。
- The research results shown that: the model obtained could well describe the process of penicillin fermentation in fed batch, as the network architecture is 5 3 5 3 topology, learning step is 1, initial weight is 0.98 and convergence rule is 10 10 . 研究结果表明：当网络拓扑结构为5?3?5?3，步长为1、初始权值取0?98及收敛准则为10?10时，所得到的模型能够很好地描述青霉素补料分批培养过程
- Fed batch fermentation can also increase 1,3 PDO concentration and yield,accompanying the maximal 1,3 PDO concentration and yield,397.7 mmol/L and 0.625 mol/mol is got respectively. 采用流加甘油发酵工艺 ;1 ;3 丙二醇浓度和转化率分别可提高到 397.;7mmol/L和 0
- When the fed batch reaction was made under the above conditions, our results obtained were that concentration of FDP accumulation was 0.399mol/L(135.65g/L) and yield in terms of the glucose consumption was 28.2%. 在该条件下，于补料分批式反应中，FDP积累浓度达到0.;399mol/L（135
- During the small vessel culture,batch culture and feeding culture in the fermenter,the characteristics of cell growth and metabolism of oxygen resistant Bifidobacterium bifidum were discussed. 分别讨论了耐氧两歧双歧杆菌在小瓶培养、发酵罐间歇培养以及流加培养的生长代谢特性,探讨了实现其高密度培养的途径。
- The effects of carbon-nitrogen mass ratio (C/N) on growth and hydrogen production were examined by batch culture with a strain of fermentative hydrogen producing bacterium Rennanqilyf3. 利用间歇培养研究了碳氮质量比对纯培养制氢工程中发酵产氢细菌产氢性能的影响。
- Unwaged members pay a lower entrance fee. 无固定收入的人入场费从优。
- That boxer is a prodigious feed. 那个拳击家是个大肚汉。
- Study on the feed batch culture technology of Bt submerged fermentation 苏云金杆菌深层发酵补料分批培养工艺研究
