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- The recent development of fuel ethanol industry was introduced in this paper and the research progress of various ethanologenic recombinant enteric bacteria was reviewed. 本文介绍了燃料酒精工业发展的最新动态,并对多种产乙醇重组肠道细菌的研究进展作了综述。
- ethanologenic recombinants 产乙醇重组菌
- The ratio of the recombinants which formed transparent zones on the Congo Red plates was 23.5%. 重组子经刚果红平板验证:约有23.;5%25菌落呈现透明圈;
- We have successfully obtained the transgenic potatoes which expressed the PBI/hvp7 recombinants. 成功地构建了重组PBI12 1 hvp7质粒 ,获得表达外源基因vp7的转基因马铃薯
- The positive recombinants were identified, cloned, sequenced and analysed with software and internet. 利用软件和互联网对核酸序列进行分析 ,确定目的基因。
- There are at least nine HIV subtypes in total, and several recombinants of these. 目前至少有9种艾滋病病毒亚型,还有这几种亚型的重组类型。
- The resultant recombinants were transformed into XL-10 GOLD super strain cells for further replication. 将阳性同源重组腺病毒载体转化到XL-10 GOLD超级感受态细胞进行扩大培养。
- Clarke-Carbon equation can give a good estimation for the necessary number of recombinants in a gene library. Clarke-Carbon公式用于估计一个完整的基因文库所需的最少克隆数。
- Although desirable recombinants were recovered in the progenies as expected, they generally appeared to be weak and lack of vigorous plant growth. 如所预料,重组的基因型出现于后裔,但这些品系多数显示软弱缺乏旺盛的生长力。
- Western blots analysis with the HMW glutenin subunit specific polyclonal antibody exhibited the expression of the recombinants in E. coli. 利用高分子谷蛋白亚基的特异性多克隆抗体,Western杂交实验证实高分子谷蛋白亚基编码基因的PCR介导重组产物可以进行原核表达。
- Recombinants appear when a mate is unfaithful and links up genes that were never meant for each other.Recombinants did not, however, create genes. 当互补子出错,接起两段不该在一起的基因时,就会出现重组体,然而重组体仍不能创造基因。
- Most of recombinants had satisfied growth, spore production and pathogenicity ability, indicating that asexual recombinants had high fitness. 大多数无性重组体都有较好的生长、产分生孢子能力和致病力,表明无性重组体有较高的适合度。
- Enzymes cutting confirmed the positive directed ligation between AADC gene and pCDNA3. The recombinants were expressed successfully in primary muscle cells. 酶切证实基因片断正向连接于pCDNA3上,并在原代骨骼肌细胞中获得表达。
- Subsequently, four recombinants were transfected into H22 with Lipofectamine Reagent, then G418-resistence colonies were acquired, and named H22-CD80+ ? H22-CD86+? H22-CD137L+ ? 用阳离子脂质体将重组子转染H22,经G418筛选,获得阳性克隆,分别命名为H22-CD80+细胞、H22-CD86+细胞、H22-CD137L+细胞、H22-CD80/CD86+细胞和H22-CD80/CD86/CD137L+细胞。
- Schmaljohn CS,Chu YK,Schmaljohn AL,et al.Antigenic subunits of hantaan virus expressed by baculovirus and vaccinia virus recombinants[J].J Virol,1990,64:3162. 尹文;徐志凯;阎岩;等.;汉坦病毒S基因的分段表达及表达产物的鉴定[J]
- The recombinants characterized in this study had a structure similar to their corresponding natural genes, although with a significantly different central repetitive domain. 序列比较显示本研究得到的PCR介导重组产物与天然高分子谷蛋白亚基具有相同的基本结构,但它们中间重复区的差异相当大。
- The fragments of 504bp and 417bp were respectively amplified by PCR and RT-PCR from Brachinorus plicatilis. 3.Seven recombinants of PCR and RT-PCR were sequenced. 采用PCR和RT-PCR方法,从褶皱臂尾轮虫基因组DNA及其总RNA中分别扩增到大小为504bp和 417bp的片段。
- Joyce and Lincoln, for example, noticed in their postmortem examination of the experiment that the three most successful recombinants had formed a clique. 毕竟从分子层面来看,生物演化仍有一些难解的谜题。
- Objective To observe the theraputic effect of grafting the primary cultured skeletal muscle cells modified by eukaryotic expression recombinants of aromatic-l-amino-acid decarboxylase (AADC) gene into the striatum of Parkinsonian SD rats models. 目的 观察人类神经元性芳香族氨基酸脱羧酶 (AADC)基因修饰的原代培养SD大鼠骨胳肌细胞脑内移植后对帕金森病模型鼠的治疗效果。
- Results: CVB3m eDNA expression library wasconstructed , and the cloning capacity was 3.18 X 106 recombinants, the titration ofpackaged phage was up to 1.59X ~ and the inserted proportion accounted for 71%. 所构成的CVB_(3m)cDNA文库的库容量为3.;18×10~6重组子,噬菌体包装液滴度为1