Subsequently, four recombinants were transfected into H22 with Lipofectamine Reagent, then G418-resistence colonies were acquired, and named H22-CD80+ ? H22-CD86+? H22-CD137L+ ?

• 用阳离子脂质体将重组子转染H22，经G418筛选，获得阳性克隆，分别命名为H22-CD80+细胞、H22-CD86+细胞、H22-CD137L+细胞、H22-CD80/CD86+细胞和H22-CD80/CD86/CD137L+细胞。