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- enzyme restriction analysis 酶限制分析法
- The main methods and results are as follows: 1.The constructed expression vector, pMSCV-ncam, was constructed and proved correct by enzyme restriction pattern. 2. 实验方法结果如下： 1.;对已构建含有NCAM的质粒载体进行酶切鉴定，证明表达载体构建正确，转化入细菌(TOP10)，扩增，纯化，备用。
- The enzyme restriction sites were added at the head of ATG of the two genes considering the express of the prokaryotic plasmid pET-28a(+) and the eukaryotic plasmids pcDNA3.1(+) and pEGFP-C3 when designing the primers of the two genes. 引物设计时，根据原核表达载体pET-28a(+)和真核表达载体pcDNA3.;1(+)、pEGFP-C3表达的需要，在基因起始密码子ATG前加入酶切位点的同时，注意补加碱基，以确保不发生读码框移。
- Restriction analysis and DNA sequencing showed that the recombinant plasmid contained the coding region of human full length MUC1 gene. Transfection experiment verified that MUC1 gene could be expressed in COS 7 cells. 酶切鉴定和序列分析证实 ;重组质粒含有人MU C- 1全长 c DNA编码序列 ;转染实验表明 MU C- 1基因能在 COS- 7细胞中表达 .
- Sequence and restriction analysis revealed APN gene was cloned in frame into pGEX-4T,SDS-PAGE profile showed a clear protein band with a relative molecular weight of 135 000. Conclusion:Human APN gene was successfully cloned ? 重组质粒测序和酶切结果显示 :APN基因已正确插入 pGEX 4T ,重组蛋白及纯化产物SDS PAGE在 135 0 0 0处有一条明显的蛋白表达条带。
- The satisfied multiple fingerprints were obtained by this template DNA for both ARDRA (amplified ribosomal DNA restriction analysis) and RISA (ribosomal intergenic space analysis). 用该方法提取的基因组DNA做模板，进行扩增核糖体限制性酶切片断分析(ARDRA)及核糖体基因间区序列分析(RISA)，均获得了理想的多态性指纹图谱；
- Amplified ribosomal DNA restriction analysis 限制性片段长度多态性分析（ARDRA）
- Combined bisulfite restriction analysis (COBRA) 重亚硫酸钠处理限制性酶切图谱分析
- amplifed ribosomal DNA restriction analysis 扩增性rDNA限制性酶切片段分析
- The Restriction Enzyme Database from NEB. 限制苺资料库取自新英格兰生物实验室公司。
- Capacity Restriction Analysis on Semi-final Grinding System 影响半终粉磨系统产量的因素简析
- confirm orientation by restriction analysis with EcoR I and BamH I. （2）EcoR和 BamH酶切鉴定。
- I restrict myself to smoking two cigarettes a day. 我限定自己每天只抽两支烟。
- One who draws blood for analysis or transfusion. 放血医师为化验或输血而抽血的人
- RESTRICTION ANALYSIS OF HEPATITIS B VIRUS GENOME(daw) ISOLATED IN CHINA 我国乙型肝炎adw亚型基因组酶切图谱分析
- Your critical analysis helped me a great deal. 你的评论分析对我帮助很大。
- The metal was submitted to analysis. 对该金属进行了分析。
- Recent laws have tended to restrict the freedom of the press. 新法例有限制新闻自由的趋势。
- Her analysis was correct to the nail. 她的分析极其正确。
- An enzyme that catalyzes genetic recombination. 催化重级酶能催化基因重组的一种酶
