The enzyme restriction sites were added at the head of ATG of the two genes considering the express of the prokaryotic plasmid pET-28a(+) and the eukaryotic plasmids pcDNA3.1(+) and pEGFP-C3 when designing the primers of the two genes.

• 引物设计时，根据原核表达载体pET-28a(+)和真核表达载体pcDNA3.；1(+)、pEGFP-C3表达的需要，在基因起始密码子ATG前加入酶切位点的同时，注意补加碱基，以确保不发生读码框移。