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Cells after twice adhesions to culture flask were collected and labeled with CD14 and CD4.A is isotype control, B shows CD14 expression ratio (85.7%). 通过流式细胞仪检测分析单核细胞CD14标记显示，本研究中单核细胞的分离富集效率为76%25-88%25，图1为1次单核细胞分离后流式细胞仪检测单核细胞标记CD14和CD4的表达情况。
When the cells proliferated and completely covered the bottom of culture bottle, passage culture was performed. 用抗半乳糖脑苷脂抗体和抗胶质原纤维酸性蛋白抗体进行细胞分类免疫组化鉴定。
The control system drives the robot move to the default position where scions have been prepared to plant, and the end-effector grip and transplant the scions into a culture flask. 机器人能自动定位于预设之取苗及挿植位置，使其手端得顺利夹持及挿植组织培养苗；
Primary MSCs covering the bottom of culture bottle were washed once with D-Hanks liquid,and digested for 4 minutes with 0.25% trypsin. Then LG-DMEM was used to collect and deposit the cells at 1 000 r/min for 10-minute centrifugation. 兔骨髓间充质干细胞的克隆化:将铺满培养瓶底的原代骨髓间充质干细胞;用D-Hanks液小心的洗1次;加入0.;25%25胰蛋白酶消化约4min;弃消化液;加LG-DMEM培养液收集细胞;1000r/min;离心10min;然后用LG-DMEM培养液充分混匀沉淀细胞。
culture flask (Kolle Roux), of glass 长颈玻璃培养瓶（科勒瓶、鲁氏瓶）
Development of Culture Flask of Sulfur Bacteria and Its Application 硫细菌培养瓶的研制及应用
Agitation can also be achieved by placing culture flasks on reciprocating or gyrotory platform shakers. 搅拌同样能够在放置在往复作用振荡器或是电流变相器平台搅拌器上的培养烧瓶中完成。
Meanwhile, culture bottles sealed with cotton plug and single closure could keep humidity of the inner bottles and was benefit for micrografting. 采用不同封口材料控制培养瓶内湿度的研究表明，棉塞和单层封口膜封口可保持瓶内湿度，并有利于微嫁接苗的成活。
EFFECT ANALYSIS OF BIPHASIC BLOOD CULTURE BOTTLE APPLIED IN CLINIC 双相血培养瓶的临床应用效果分析
Evaluation on Re-using Experiment of Automatic Blood Culture bottle 全自动血培养瓶的再利用实验评价
The system uses an infrared sensor to monitor the spawn in the quantitative plate, the blockage of the spawn discharger, and the spawning operation into the culture bottles. 其主要技术内容系利用红外线感测装置检测定量盘中有无菌种及是否接种于培养瓶中，以达成菌种瓶中菌种用罄或菌种定量输出器是否堵塞需要清理之即时预警；
The flask culture process was analysed. 同时对摇瓶发酵过程进行了分析。
Naturalization of a new culture is always faster among the young. 对新文化的采纳在年轻人中总是快一些。
Obtainment and sequencing of the cDNA derived from the non-structural P2C of CVB4 First,we inoculated CVB4 into Hela cells which had been cultivated and spreaded the bottom of culture bottles. 一、柯萨奇B4病毒非结构蛋白P2C CDNA的获取首先采用细胞培养技术，当人宫颈癌细胞（HeLa ）长满后,接种柯萨奇B4病毒，细胞病变达到80%25时及时收获病毒。
Determination of Bacterial Cell Population for Hydrocarbon-Degrading Bacteria by Using Culture Flask Test 细菌瓶法用于石油烃降解菌菌数测定
He was one of the apostles of the new culture. 他曾是新文化的倡导者之一。
Determination of Bacterial Cell Population for Xanthan Degrading Bacteria by Using Culture Flask Test 细菌瓶法用于黄原胶降解菌菌数测定
with EBM-2 adding EGM-2 MV in the 25 cm~ 2 culturing flask in the fourth group. 第4组用添加有EGM-2MV的EBM-2在25cm2细胞培养瓶中培养。
Greece, the cradle of Western culture. 希腊，西方文化的发源地。
Greek culture penetrated Persia through the medium of the Syrians. 通过叙利亚人作媒介，希腊文化渗透到波斯。