Obtainment and sequencing of the cDNA derived from the non-structural P2C of CVB4 First,we inoculated CVB4 into Hela cells which had been cultivated and spreaded the bottom of culture bottles.

• 一、柯萨奇B4病毒非结构蛋白P2C CDNA的获取 首先采用细胞培养技术，当人宫颈癌细胞（HeLa ）长满后,接种柯萨奇B4病毒，细胞病变达到80%25时及时收获病毒。