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- cerb-B1基因 cerb-B1 gene
- 说明本研究构建的针对cerb-B1基因的质粒表达载体可以显著抑制cerb-B1基因和EGFR蛋白在人结肠癌LoVo细胞的表达,为结肠癌的基因治疗提供了新的思路。 The plasmid expression vector aiming directly at cerb-B1 gene might suppress cerb-B1 gene and EGFR expression in human colon cancer LoVo cells. The new therapeutic modalities could be used in the treatment for human colon cancer.
- 目的:体外构建编码人类基因cerb-B1短发卡状RNA(shorthairpinRNA,shRNA)的质粒表达载体,观察其对结肠癌LoVo细胞cerb-B1基因和表皮生长因子受体(EGFR)蛋白的特异性抑制作用。 Objective:To establish a plasmid expressive vector coding short hairpin RNA(shRNA) of human cerb-B1 gene in vitro, and to investigate specific inhibition of cerb-B1 gene and epidermal growth factor receptor(EGFR) expression in human colon cancer LoVo cells by shRNA.
- B1基因 B1 gene
- hUNC93-B1基因 hUNC93-B1 gene
- Cerb-B-2 Cerb - B - 2
- 细胞周期素B1基因 cyclin B1
- 基因代代相传。 Genes are transmitted from one generation to another.
- 应用脂质体Lipofectamine2000转染人结肠癌LoVo细胞,转染成功后以G418筛选4周,实时荧光定量RT-PCR(RealTimeRT-PCR)检测cerb-B1基因的mRNA表达,Western-blot检测EGFR蛋白的表达。 The human colon cancer LoVo cells were transfected by Lipofectamine 2000 with plasmid vector, and then selected for 4 weeks by G418. The expression of cerb-B1 mRNA was assessed by real time PCR and the protein of EGFR was assessed by Western blot method.
- Wx-B1 Wx-B1
- 基因外显率 penetrance of gene
- V．B1 维生素B1, 硫胺素, 硫胺, 维生素乙1, 维他命乙1, 抗神经炎素[维生素类药]
- 分离的基因型 a segregant genotype.
- 基因的区分繁殖 differential perpetuation
- Ｃ基因 C gene
- Ｄ基因 D gene
- Ｊ基因 J gene
- Ｖ基因 V gene
- Ｉｒ基因 Ir gene
- 免疫遗传学免疫学中研究免疫反应的细胞和基因基础的分支 The branch of immunology that deals with the molecular and genetic bases of the immune response.
