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- According to the published canine parvovirus(CPV) VP2 gene in GenBank,a pair of primers was designed and synthesized to amplify the VP2 gene. 根据GenBank上发表的犬细小病毒(CPV)VP2蛋白基因序列,设计并合成1对引物,通过PCR扩增VP2基因全长。
- Methods:The authors acquired VP2 gene by PCR amplifing method,and insert VP2 gere into PUC119 plasmid by cloning for sequencing. 方法 :采用PCR方法获得鸡贫血病毒的VP2基因 ,采用平端连接将其克隆到PUC119载体上进行测序 ,采用粘端连接将其亚克隆到PET载体上并进行原核表达。
- To predict antigen epitopes on B cell of VP2 of canine parvovirus 2b subtype(CPV-2b),the VP2 gene of CPV-2b was cloned and sequenced. 对犬细小病毒2b亚型衣壳蛋白VP2基因进行了克隆、测序,并用分子生物学软件DNAStar对VP2基因的推导氨基酸序列进行了表位分析。
- Standard positive template constructing A pair of primers was designed according to the VP2 gene of PPV sequence in GenBank. 2．标准阳性模板的制备 根据GenBank中猪细小病毒VP2基因序列设计一对引物，用PCR从PPV细胞培养物中扩增出目的基因（98 bp）。
- The pCVP21 was contructed by inserting the amplified VP2 gene fragment into expression vector pCYTEXP1, containing bacteriophage promoters P R and P L in tandem preceded by the cIts 857 repressor gene. 扩增产物经双酶切后插入含噬菌体P?RP?L双强启动子的pCYTEXP1表达质粒，构建了VP2基因克隆pCVP21，经Dot?ELISA筛选出4个VP2表达阳性克隆。
- VP2 Gene of Porcine parvovirus (PPV) SD-68 strain was cloned and sequenced . The results showed that VP2 Gene of Porcine parvovirus (PPV) SD-68 strain contained 1740bp and encoded a protein of 579 amino acids . 对猪细小病毒(PPV)SD-68株VP2基因进行的克隆和序列测定表明:SD-68株VP2基因全长1740bp,编码579个氨基酸残基组成的多肽;
- Apair of primers was designed to amplify VP2 gene by PCR according to the published sequence of CPV's VP2 gene with primer5.0 software. The product of PCR named VP2 is approximate 1.8kb in length. 根据Genbank已发表的CPV-2 VP2 基因的序列;利用primer 5.;0 软件设计并合成一对引物;通过PCR 扩增出长约1
- Cloning and Sequence Analysis of the VP2 Gene of CPV Nanjing Isolate, CPV-GNThe present studies involve T-A cloning of major capsid- subunit-encoding VP2 gene of canine parvovirus Nanjing isolate, CPV-GN. 本研究涉及CPV-GN毒株VP2基因的克隆与序列分析。
- The NS1 gene and VP2 gene of porcine parvovirus (PPV) of HN1-strain were amplified by polymerase chain reaction (PCR). The amplified NS1 fragment of PPV was 624bp in length ; the amplified VP2 fragment of PPV was 882bp in length . 本研究利用PCR技术对猪细小病毒HN1株NS1基因与VP2基因进行扩增,分别得到624bp和882bp的基因片段。
- The VP1 and VP2 genes, under the control of SV40 promoter and CMV promoter respectively , were ligated and inserted into the gC gene region of herpesvirus of turkey (HVT) . 通过同源重组技术 ,将目的基因插入到火鸡疱疹病毒 (HVT)gC基因区 ,构建了一株含CIAVVP1、VP2基因表达单元的重组HVT(VP1VP2_rHVT)。
- VP1 and VP2 genes of chicken anaemia virus were cloned into transfer vector pBacPAK8, then recombinant transfer plasmids pBac vp1 and pBac vp2 were obtained, respectively. 将鸡贫血病毒 VP1和 VP2基因分别克隆入转移载体 p Bac PAK8中 ,获得重组转移质粒 p Bac-vp1和 p Bac-vp2。
- hypervariable region of VP2 gene VP2蛋白基因高变区
- Cloning and sequencing of VP2 gene of PPV BM-1 strain 猪细小病毒VP2基因的克隆与序列测定
- Cloning,sequencing and expression of VP2 gene of CAV SJ1 strain 鸡贫血病毒VP2基因的克隆、序列分析及表达
- Molecular Cloning and Antigenicity Analysis of the VP2 Gene of PPV 猪细小病毒NJ-1株VP2基因克隆与抗原性分析
- Keywords Canine parvovirus（CPV）;VP2 gene;Sequence analysis; 关键词犬细小病毒;VP2基因;序列分析;
- Keywords CPV VP2 gene;Pichia pastoris;Eukaryotic expression; 犬细小病毒VP2基因;毕赤酵母;真核表达;
- The position that a given gene occupies on a chromosome. 基因座在一条染色体上某种给定基因所占的位置
- Half of the people who inherit the gene express it. 半数有这种遗传基因的人，皆显示出其影响
- Studies on Inhibitory Factors of Proliferation in Vitro, Variation of VP2 Gene and DNA Vaccine of Porcine Parvovirus 猪细小病毒体外增殖的抑制因素、VP2基因变异及核酸疫苗的研究
