Methods:The authors acquired VP2 gene by PCR amplifing method,and insert VP2 gere into PUC119 plasmid by cloning for sequencing.

• 方法 :采用PCR方法获得鸡贫血病毒的VP2基因 ,采用平端连接将其克隆到PUC119载体上进行测序 ,采用粘端连接将其亚克隆到PET载体上并进行原核表达。