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- T7RNA聚合酶表达系统 T7 RNA polymerase expression system
- T7RNA聚合酶 T7 RNA polymerase
- 自H5N1阳性病料中提取总RNA,反转录后采用高可信度DNA聚合酶,经PCR扩增NP基因,采用Invitrogen定向表达系统进行克隆表达,纯化获得N末端携带多聚组氨酸标签的重组NP蛋白,分子质量约66.0ku。 Total RNA was extracted from positive clinical sample of H5N1 subtype of avian influenza virus, NP gene was amplified by reverse transcriptase-polymerase chain reaction using high proofreading polymerase (PyobestTM DNA Polymerase), and expressed using Invitrogen championTM pET directional TOPO expression system. Recombinant protein containing polyhistidine (6xHis) tag in N-terminal was about 66.0 ku in size, had been obtained and purified.
- ＤＮＡ聚合酶 DNA polymerase
- T4DNA聚合酶 T4DNA polymerase
- 淋巴系统 lymphatic system
- 磷酸酶 phosphatase
- II型系统 type-II system
- ０Ｌ系统 zero-sided Lindenmayer system
- Ｔ系统 transverse tubular system
- (使)聚合 polymerize
- Ｑ酶 branching enzyme
- ２Ｄ系统 2D system
- 高聚合凝聚剂 high polymer coagulant
- ＭＫ系统 MK system
- [生化]合酶 synthase
- 磷酸聚合 phosphoric acid polymerization
- ＤＮＡ酶 deoxyribonuclease
- ＲＣ系统 Ritchey-Chretien system
- ＲＮＡ酶 ribonuclease
