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Reverse blot hybridization 反向点杂交
The 16S rRNA PCR-membrane reverse dot blot hybridization technique showed that the sensitivity was 92.49%, and the specificity was 100%. PCR-膜反向斑点杂交技术鉴定分枝杆菌菌种的灵敏度为92.;49%25;特异度为100%25。
Effect of labeling was detected by dot blot hybridization. 点杂交方法检测探针标记效果。
reverse cross blot hybridization 反向杂交
We detected 213 specimens from 173 people using probe of pBR-322 by Dot and Southern blot hybridization. 我们用常见的克隆载体pBR322作探针,用核酸点杂交和Southern吸印方法,检测了来自173人的213例样品。
Objective: To study the clinical value of detecting mycobacterium tuberculosis by dot blot hybridization. 摘要目的：探讨斑点杂交法检测结核分枝杆菌的临床应用价值。
From recombinant plasmid pGEMTH2 prepared cRNA probe was identified by dot blot hybridization. 使用斑点杂交证实我们制备的探针是敏感而可靠的。
To develop a universal PCR-reverse blot hybridization assay, which aime to detect and identify pathogenic yeast rapidly and exactly. 摘要建立PCR结合寡核苷酸探针反向斑点杂交技术，快速检测及鉴定致病性酵母菌。
Methods:Polymerase chain reaction in combination with dot blot hybridization of allele specific oligonucleotide(PCR/ASO) probes was used. 方法：采用聚合酶链反应结合等位基因特异的寡核苷酸探针杂交（PCR/ASO）技术。
Methods:Using digoxigenin-labeled probe,75 sera of known or unknown etiology, and 17 blood donors were detected by dot blot hybridization. 方法：用地高辛素标记基因探针，斑点杂交法检测未知和已知病原的肝炎患者75例，正常对照17例。
Expression of messenger endothelin 1 (ET 1) RNAs and its receptor subunits (ETA,ETB)were studied by northern blot hybridization in rat kidney following renal ischemia reperfusion. 为了探究内皮素?1（ET?1）对肾功能的影响和作用方式，采用斑点杂交和原位杂交方法对大鼠缺血60分钟再灌注肾组织ET?1及其受体亚型（ETA、ETB）的基因表达进行了研究。
Methods: The gene rearrangement in blood and bone marrow of 12 children with AML was detected by Southern blot hybridization using LE11. 8, MYO and M13 probes. 方法：用LE11.;8、MYO和M13探针，经Southern杂交法检测12例儿童急性粒细胞白血病患者的外周血或骨髓细胞的基因重排。
Methods b-FGF expressions were examined by RNA dot blot hybridization and immunohistochemical staining in specimens of 40 normal prostate tissues(NP)and 38 BPH tissues. 方法采用斑点杂交技术和免疫组化染色测定40例正常前列腺(NP)、38例前列腺增生症(BPH)组织中b-FGF的表达情况。
The purpose of this study was evaluation of RT PCR technique,dot blot hybridization with PCR generated probe and SDS PAGE for the detection of rice black streaked dwarf ?Fijivirus?(RBSDV). 用RT PCR技术、PCR标记的探针点杂交和SDS PAGE检测了生产上严重危害玉米和水稻的水稻黑条矮缩病毒(RBSDV)。
After incubation with Cpd 861 for 48 hours, HSC was harvested to determine collagen type I,III,IV mRNA by dot blot hybridization and cell culture medium was used to detect collagen secretion by ELISA method. 传一代细胞以10mg/ml复方861作用48小时后 ,以ELISA法、斑点杂交法检测胶原蛋白分泌及相应mRNA水平的变化。
The FMR-1 gene mutation and Xq27.3 fragile site among 233 non-specific mentally retarded children were investigated in our genetic counseling department and two special educational schools by PCR, Southern Blot hybridization and cytogenetic methods. 采用PCR、Southern Blot印迹杂交及细胞遗传学方法;对233名原发性智力低下患儿进行了FMR-1基因的突变分析和Xq27.;3脆性位点检查。
Dot blot hybridization with the two probes on Nitrocellulose Membranes showed the positive results for PPV DNA from different resources and PUP recombinant plasmid,but negative for the nucleic acid samples obtained from a series of control virus. 分别对不同来源的猪细小病毒DNA及PUP重组质粒于硝酸纤维素膜上打点杂交，免疫呈色后均为阳性反应，而对照的猪瘟病毒、乙型脑炎病毒、伪狂犬病毒、PK－15细胞的核酸均为阴性反应。
Dot blot hybridization with the two probes showed the positive result for PPV DNA,but negative for the nucleic acid samples obtained from Hog cholera virus,Pseudorabies virus,Japanese B Encephalitis virus and PK 15 cells. 对猪细小病毒DNA进行斑点杂交，两种探针均为阳性，而对照的猪瘟病毒、猪伪狂犬病毒、乙型脑炎病毒及PK－15细胞的核酸均为阴性。
Our army suffered a slight reverse. 我们的部队受到轻微的挫败。