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- The vector was identified via PCR and restriction enzyme analysis and was introduced into Agrobacterium tumerifacience LBA4404. 经限制性内切酶分析和PCR鉴定后利用冻融法将重组质粒pBI121-A导入根癌农杆菌LBA4404。以锦橙 (Citrus.
- Methods: The serum samples were collected from 56 patients with morbilliform maculopapular eruption.The target DNA of CBV was detected by RT-PCR and restriction enzyme analysis. 方法:取56例春季流行性麻疹样发疹患者外周血标本,扩增目的DNA并进行特定限制性内切酶分析。
- Based on the analysis results, three partition of VP1 gene of CAV were cloned and inserted into the bacterial plasmids pGEX-4T-1. The recombinant plasmids containing VP1 partiton gene of CAV were identified by restriction enzyme analysis and PCR method. 然后将VP1基因分段克隆至原核表达载体pGEX-4T-1，经酶切及PCR鉴定，证明成功构建了重组表达载体pGEX-A、pGEX-B、pGEX-C。
- Methods HSP70 cDNA was inserted into polylinker sitr of eukaryotic expression vector pcDNA 3.1 to construct pcDNA AHSP70, in which restriction enzyme analysis was used to confirm the reverse orientation of the HSP70 cDNA from the individual transformants. 方法 将HSP70cDNA反向克隆入 pcDNA 3.;1;构建CMV启动子控制的真核表达载体 pcDNA AHSP70;用酶切鉴定结果 ;
- restriction enzyme analysis (REA) 限制性内切酶分析技术
- Polymerase chain reaction and restriction enzyme analysis of human cytomegalovirus infection in pregnant women and neonates 孕妇及新生儿巨细胞病毒感染的聚合酶链反应及限制酶分析
- Detection of Human Cytomegalovirus Infection in Pregnant Women and Neonates by Nested Polymerase Chain Reaction with Restriction Enzyme Analysis and Virus Isolation 孕妇及新生儿巨细胞病毒感染的分子生物学诊断
- The Restriction Enzyme Database from NEB. 限制苺资料库取自新英格兰生物实验室公司。
- Restriction enzyme analysis 限制酶分析
- The Detection of Human Cytomegalovirus in Pregnant Women of the First Trimester and Neonates by Nested Polymerase Chain Reaction With Restriction Enzyme Analysis and Virus Isolation 聚合酶链反应限制酶切分析及病毒分离检测孕早期孕妇和新生儿巨细胞病毒感染
- Enzyme analysis of these Fab antibodies was accomplished for diversity. 筛选获得了结合血管内皮细胞生长因子( VEGF)的人噬菌体 Fab抗体 ;并对所获抗体的多样性进行了进一步分析 .
- It enables a specific gene to be located on a particular restriction enzyme fragment. 它就能使专一的基因被定位于特定的限制性内切酶切成的片段上。
- Based on the number of restriction enzyme detecting RFLPs, most of mutations were attributed to point mutation. 根据揭示多态性的限制性内切酶的数量可将产生的突变大多归为点突变。
- Certain DNA sequences oriented within this gap are substrates for Alu I, a blunt end restriction enzyme. 如果用一段酶切断作为桥梁的DNA，那么整个电路的电流也被中断了。
- Amplified of the green fluent protein (GFP) gene from plasmid PCR3.1, and add suitable restriction enzyme cutting site. 从含有gfp基因的质粒pCR3.;1上扩增出全长基因，并且加上合适的酶切位点。
- Results: Recombinant expression plasmid of ALR was confirmed correct by restriction enzyme digestion and sequencing. 结果：酶切鉴定及测序结果提示表达产物正确；
- ECL is widely used in biosensor, immune sensor, DNA probe, surface analysis and enzyme analysis. ECL在生物传感器研究、免疫分析和DNA探针、表面分析和酶分析中得到了广泛的应用。
- The expressive vector pcDNA3.1(-)-ASL has been constructed and had been confirmed by restriction enzyme cut and sequencing. 构建的真核表达载体pcDNA3 .;1(-) ASL经过酶切鉴定和测序证实正确。
- Genomic DNA was extracted.Polymerase chain reaction, direct sequencing and restriction enzyme reaction were performed to identify the mutations. 利用聚合酶链反应、DNA直接测序进行突变检测,进一步采用限制性内切酶酶切验证突变。
- Restriction enzyme digestion and AT clone were employed to rapidly screen out the recons of PA segments for the preparation of the gene chip probes. 利用酶切、AT克隆方法快速分析筛选出保护性抗原基因片段的重组子,从而制备成芯片探针。
