Based on the analysis results, three partition of VP1 gene of CAV were cloned and inserted into the bacterial plasmids pGEX-4T-1. The recombinant plasmids containing VP1 partiton gene of CAV were identified by restriction enzyme analysis and PCR method.

• 然后将VP1基因分段克隆至原核表达载体pGEX-4T-1，经酶切及PCR鉴定，证明成功构建了重组表达载体pGEX-A、pGEX-B、pGEX-C。