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- Antigen was appraised by peptide mass fingerprint (PMF) matched with Mascot human protein database. PMF was obtained by immunoprecipitation and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). 用免疫沉淀和基质辅助激光解吸电离飞行时间质谱(matrix-assistedlaserdesorption/ionizationtime-of-flightmassspectrometry,MALDI-TOF-MS)得到相应的肽质量指纹谱(peptidemassfingerprint,PMF),再通过Mascot在人类蛋白质数据库中分析比对鉴定mAb对应的抗原。
- Selecting differentially expressed proteins to march enzymolysis in gel, detect peptide mass fingerprint by MALDT-TOF-MS, then research data base of Swiss-port to identify the proteins. 选取差异蛋白质点进行胶内酶解,以MALDI-TOF-MS测肽质量指纹图,检索Swiss-Port数据库,对蛋白质进行鉴定。
- The facility also provides computer termini for peptide mass fingerprinting (PMF) and/or MS/MS ions searching against database. 同时也提供电脑及资料库供使用者做资料搜寻之用。
- MALDI-TOFMS has been established as powerful tools for the identification of proteins by peptide mass fingerprinting in proteomics. 由于其信号检测灵敏、速度快以及种类多样化的优点，生物质谱已经成为生物分析领域中最前沿研究如蛋白组学研究的支撑技术。
- Keywords Two-dimensional electrophoresis;Mass spectrometry;Peptide mass fingerprint;Epididymis;Proteome; 双向凝胶电泳;质谱;肽质量指纹谱;附睾;蛋白质组;
- Application of Peptide Mass Fingerprint in Ricin Identification with Matrix-assisted Laser Desorption Ionization Time-of-Flight-Mass Spectrometry 一种基于肽质量指纹谱技术鉴定蓖麻毒素的新方法
- Keywords Ricin;peptide mass fingerprint;matrix-assisted laser desorption ionization time-of-flight-mass spectrometry;biomass spectrometry; 蓖麻毒素;肽质量指纹谱;基质辅助激光解吸电离飞行时间质谱;生物质谱;
- Keywords hybridoma;peptide mass fingerprinting;chimeric antibody; 杂交瘤;质谱分析;嵌合抗体;
- Peptide mass fingerprint 肽质量指纹谱
- peptide mass fingerprint search 肽质量指纹谱搜索
- Keywords Somanase;Peptide Mass Fingerprinting;Squid-type DFPase;Active site; 人肝梭曼水解酶;肽指纹图谱信息;鱿鱼型的DFP酶;活性位点;
- Keywords apple juice concentrate;protein;peptide mass fingerprinting;amino acid analysis; 浓缩苹果汁;蛋白质;肽质量指纹谱;氨基酸分析;
- Keywords esophageal cancer;proteomics;mass spectrometry;peptide mass fingerprinting;RT-PCR; 食管癌;蛋白质组;质谱分析;肽质量指纹图;RT-PCR;
- Keywords Biological sample;analysis of protein drug;endostatin;bio-mass spectrometry;peptide mass fingerprinting （PMF）;tandem mass spectrometry; 生物样品;蛋白质药物分析;内皮抑制素;生物质谱;肽质量指纹谱;串联质谱;
- Keywords myasthenia gravis;muscle skeletal;P25 protein;immunolocalization;Peptide Mass Fingerprinting;carbonic anhydrase III; 重症肌无力;骨骼肌;P25蛋白;免疫定位;肽指纹图谱;碳酸酐酶III;
- Keywords Parathyroid Hormone;Osteoblast;Two-dimensional Gel Electrophoresis（2-DE）;Proteome;Peptide Mass Fingerprinting （PMF）; 关键词甲状旁腺激素;成骨细胞;双向凝胶电泳;蛋白质组;肽质量指纹图谱;
- Keywords proteome;two-dimensional gel electrophoresis;keratinocyte;Fibroblast;matrix-assisted laser desorption/ionization mass spectrometer;Peptide Mass Fingerprinting; 蛋白质组;双向凝胶电泳;角质形成细胞;成纤维细胞;基质辅助激光解吸电离飞行时间质谱;肽质量指纹图谱;
- Peptide mass fingerprinting 肽质量指纹谱技术
- A cloud is a mass of vapor in the sky. 云是天空中的水汽团。
- MALDI-TOF MS is used to identify proteins by matching a list of experimental peptide masses with the calculated list of all peptide masses of each entry in a database. 例如，未知蛋白质经蛋白质酵素裂解后，藉质谱仪可得到裂解产生之各种胜肽的分子量。它们可和资料库中已知蛋白质预测之胜肽的分子量比对，比对之结果可用以辨识该未知蛋白质。
