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- ORF2基因片段 ORF2 gene fragment
- ORF2基因 ORF2 gene
- 乙肝病毒表面抗原（HBsAg）、戊肝病毒ORF2基因片段NE_2在番茄中的表达及免疫活性 Expression and Immunoactivity of HBsAg (hepatitis B Virus Surface Antigen) and HEV-NE2 (hepatitis E Virus ORF2 Gene) in Transgenic Tomato
- 含CpG基序的PCV-2的ORF2基因真核表达载体的构建 Construction of Eukaryotic Vector Bearing ORF2 of PCV-2 and Containing CpG Motifs
- 片段 segment
- 幽门螺杆菌对甲硝唑耐药相关基因片段的筛选及克隆分析 Screening for metronidazole-resistance associated gene fragments of Helicobacter pylori by suppression subtractive hybridization
- 用脂质体介导法将pEGFPTTV2质粒DNA转染Cos7细胞,通过RT-PCR分析,证实细胞中存在ORF2基因的转录产物。 The pEGFPTTV2 was transfected into Cos7 cell line using LipoFectamine 2000, and the transcription products of TTV ORF2 gene in the transfected cells were confirmed by RT-PCR.
- M基因片段 M gene segment
- 方法通过巢式PCR,从一份兰州地区无偿献血员血清中扩增TTV病毒的ORF2基因,并克隆到pGEM-T载体中,进行核苷酸序列分析。 Methods The ORF2 gene of TTV was amplified from the serum of a blood donor in Lanzhou region by nest PCR and inserted into vector pGEM-T then,after identification by DNA sequencing,subcloned to expression vector pET22b(+).
- 白血病部分未知基因片段与公共生物信息资源的比较与分析 Analysis of some leukemia unknown genes by comparison to public bioinformation resource
- ORF2片段 ORF2
- 将两基因片段分别克隆入pMD-18T载体中，提取克隆质粒； The two fragment was fusioned and inserted into pMD-18T victor.
- 假肥大型肌营养不良症cDNA基因片段的表达及其抗血清制备 Expression of Duchenne muscular dystrophy cDNA fragment in E.coli and preparation of its antiserum
- 参照GenBank发表的PCV2BF株基因序列，设计合成一对引物，对PCV2BF株ORF2基因进行PCR扩增，产物经琼脂糖凝胶电泳分析，呈现一条约720 bp的特异条带，将其回收后克隆到pGEM T-Easy载体。 A pair of primers was designed according to the PCV2 sequence in Genbank (AF381175). The ORF2 gene, a fragment of 720 bp, was amplified by polymerase chain reaction (PCR), then cloned into pGEM-T Easy vector and identified by BamHl and Xhol digestion.
- ORF2-7基因 ORF2- 7 gene
- rDNA基因片段 16S rDNA gene frgement
- ORF2截短基因 ORF 2 truncated gene
- 特异基因片段 unique gene fragments
- 结合上述毒力因子分布情况，根据SS2的cps2J、mrp、orf2基因(virulent-associate sequence orf2)设计合成三对引物，建立三重PCR检测方法，并对PCR扩增产物进行测序、酶切、特异性及敏感性实验。 The positive detection rates of cps2/gdh+/epf+/mrp+/sly+/fbps+/orf2+ were 96%25 (24/25) and only one virulent SS2 isolate was cps2/gdh+/epf-/mrp+/sly+/fbps+/orf2+.
- 猪链球菌2型胞外蛋白因子部分基因片段的克隆表达及小鼠的免疫保护性试验 Cloning and Expression of the Fragment of Epf Gene of Streptococcus Suis Type 2 and the Immune Protection Test in Mice
