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- C DNA模板扩增。 c and the DNA template of p.
- 含有人全长CblcDNA的质粒pEFHACbl作为模板扩增Cbl基因N-端(CblN); The gene fragment encoding the N-terminal part of Cbl protein (named as CblN) was amplified by PCR using pEFHACbl plasmid encoding human Cbl as templates.
- 转录酶一种在转录过程中催化DNA模板形成RNA的聚合酶 A polymerase that catalyzes the formation of RNA from a DNA template in the process of transcription.
- 采用RT PCR技术 ,以HL 60细胞mRNA为模板扩增获得编码BPI氨基端 1 93个氨基酸 (rBPI2 1)的基因片段 (BPI60 0 ) ; BPI 600 bp gene which encode 193 amino acid fragments (rBPI 21 ) BPI N terminal was amplified by RT PCR from mRNA that were extracted from HL 60;
- PCR方法检测转基因食品时,应用改良CTAB法制备DNA模板。 DNA extract by using modified CTAB methods was suitable for PCR-based detection of genetically modified organisms in foods and animals feed.
- 扩 enlarge
- 扩增 augmentation
- 对5种转基因棉籽和3种非转基因棉籽,应用改良CTAB法和改良KIT法提取DNA模板,对35S、NOS、Bt等基因进行了检测研究,并确定了在检测Bt基因时的灵敏度。 The study of detection of 35S? NOS? Bt in 5 transgenic and 3 non-transgenic cotton seeds was carried out with DNA extraction by means of modified CTAB method and modified kit method respectively.
- 方法以质粒pEGFP-N1为模板扩增EGFP基因,EcoR I和Hind III双酶切EGFP基因序列和pET28a载体。 Methods Using plasmid as template,full-length EGFP gene was amplified. The amplified EGFP gene and pET28a vector were cleaved by EcoR I and Hind III,then ligated after purifying.
- 人脑原发性肿瘤中c-fos基因的扩增、重排及过强表达 AMPLIFICATION,REARRANGEMENT AND OVER-EXPRESSION OF c-fos GENE IN HUMAN PRIMARY BRAIN TUMORS
- 扩增性 Proliferation
- 扩增图谱 amplification map
- cDNA扩增 cDNA amplification
- 扩增条带 Amplified band
- 种质扩增 Enhancement
- 分段扩增 PCR
- RAPD扩增 RAPD
- 扩增突变 trinucleotide repeat expansion
- 扩增和缺失 expansion and contraction instability
- 单管PCR扩增 one tube PCR amplification
