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- Aus＆Perc auscultation and percussion 听诊和叩诊
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- [方法]用0.25%的胰蛋白酶经冷温两步消化皮肤,分离得到NHEK,进行体外无血清培养; 用中性红吸附试验(NRU)测定PERC对NHEK的中性红吸附减少50%的浓度(NR50值),并据此确定PERC细胞毒性试验的染毒剂量; Methods Administered dose of PERC to NHEK were determined according to neutral red uptake(NRU)assay with free serum culture in vitro.
