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- 人黑色素浓集激素1型受体真核表达载体的构建及稳定转染CHO细胞系的建立 Construction of eukaryotic expressing vector of human melanin-concentrating hormone receptor 1 and establishment of stable transfectant CHO cell line
- 稳定转染的CHO细胞系 stable transfected CHO cell line
- 稳定转染的HepG2细胞系 Stable transfected HepG2 cell lines
- 稳定转染的SHG-44细胞系 stable transfected SHG-44 cell line
- 转染VMAT2的CHO细胞对DA引起的毒性有显著的抵抗作用。 VMAT2 transgenic CHO cells can resist the toxicity of dopamine.
- 幽门螺杆菌cagA基因稳定转染胃上皮细胞及其对细胞的作用 Effect of stable transfection of gastric epithelial cellls with recombinant of Helicobacter pylori cagA gene
- 筛选出了能高效稳定表达CTLA-4/Ig融合蛋白的CHO细胞; The CHO cells that could steadily express at high level the CTLA-4/Ig fusion protein was selected for cloning.
- 逆转录病毒介导的对原代培养成年大鼠成肌细胞基因转染的研究 Study of Gene Transfection Mediated by Retrovirus in Primary Cultured Adult Rat Skeletal Myoblasts
- 转染 transfection
- 高拱坝结构稳定转异和体形优化分析理论和方法 Analysis Theory and Method of Stabilization Anomaly and Shape Optimization for High Arch dams
- 腹腔内注射TNF基因转染的LAK细胞对于腹水型肝癌小鼠的疗效 Therapeutic Effect of TNF Gene-transfected LAK Cells on the Ascitic Liver Carcinoma-bearing Mice
- 稳定转导反义增殖细胞核抗原基因对膀胱癌细胞体外增殖活性的调控作用 The regulation effects on in vitro proliferation activities of human bladder cancer cells by stable antisense proliferating cell nuclear antigen gene transfer
- GCV和CTL对HSV-TK/B7基因转染的大鼠乳腺癌细胞杀伤的体外实验 In vitro cytotoxicities of GCV and CTL to rat mammary cancer cells transfected with HSV-TK/B7 gene
- 为了排除其它粘附分子的作用 ,应用联合转染DR7或 (和 )B7分子基因的CHO细胞系作为人工抗原递呈细胞 (APC)介导混合淋巴细胞反应 (MLR)。 B7 transfected CHO cells were used as artificial antigen presentation cells (APCs) in MLR to exclude the effects of other costimulatory molecules.
- 稳定转染 stable transfection
- 基因转染的SCs gene-transfection schwann cells
- 胰岛素样生长因子1基因转染对大鼠肌腱细胞生长的促进作用 Insulin-like growth factor 1 mediated gene transfer to rat tendon cells to promote the growth of the tendon cells
- 目的构建人谷胱甘肽硫转移酶A1(GSTA1)的CHO细胞表达体系。 方法重组质粒pDNA3.1-GSTA1用lipofectamineTM2000转染CHO细胞,用G418筛选细胞的阳性克隆,并用PCR、RT-PCR和Western blot进行鉴定,测定GSTA1酶活性。 Objective To establish the Chinese Hamster Ovary(CHO) expression system for the human glutathione-S- transferase (GST) A1. Methods CHO cells was transfected with recombinant vector pDNA3.1- GSTA1 by lipofectamineTM 2000. The expression of selected CHO-GSTA1 in G418-resistant clones were determined by PCR,RT-PCR and Western blot.
- 子宫内基因转染的研究进展 Progress in utero gene transfer
- 全营养液促脂质体介导质粒DNA转染结肠直肠癌细胞的实验研究 Experimental study of total nutrient admixture promoting transfection of plasmid DNA mediated with liposomes to colorectal cancer cells
