the gene mutagenesis was used to construct MTB attenuated live vaccine such as MTB purine autotroph, ICL gene deletion,VitB 5 autotroph and undecaprenol kinase mutant.

• 利用基因诱变可构建 MTB减毒活疫苗,如MTB嘌呤营养缺陷株, ICL基因剔除株,VitB5营养缺陷株和十一萜 醇激酶基因变异株。