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- specific InDel primer pairs 特异插入/缺失分子标记
- InDel primer pairs InDel引物
- By using these two primer pairs and two times of PCR protocols, Cur. 所以使用这两对引物进行两次PCR反应可将目标菌Cur.
- Using primer pairs F1/F2, an amplified fragment was obtained from each of the genomic DNA of Cur. 引物F1/F2扩增结果:F1/F2可以从Cur.
- Leishmania DNA was detected in 33%(33/100)and 30%(30/100)human blood samples by RV1-RV2 and K13A-K13B primer pairs respectively. 这两对引物对100份无症状居民血的阳性检出率分别为33%25(33/100)和30%25(30/100)。
- AFLP markers are exploited in map construction. 140 ploymorphic bands are generated by 17 primer pairs in EcoRI/Msel and Pstl/Msel eynzem combinations. 利用EcoRI/MseI和PstI/MseI两种酶切组合,分别筛选了64种引物组合,9对来自EcoRI/MseI酶切组合的引物和8对来自PstI/MseI酶切组合的引物进行了群体分析。
- Four primer pairs ( E-AAC/M-CAG E-AAC/M-CAT, E-AAC/M-CTA, E-AGC/M-CTC ) were used to do AFLP analysis for the hybrids and their parents. 利用4对选择性扩增引物(E-ACC/M-CAG,E-AAC/M-CAT,E-AAC/M-CTA,E-AGC/M-CTC)对杂交子代及其亲本进行了AFLP分析。
- Primer pairs of B1/B2, E1/E2 and 11/12 amplified of a unique 387bp, 390bp and 350bp fragment respectively from genomic DNA samples strain of Cur. 引物B1/B2、E1/E2和I1/I2扩增结果:B1/B2、E1/E2和I1/I2能分别从目标菌Cur.
- Results: All primer pairs except the No.16 could distinguish wild, asexually propagated and sexually propagated individuals of Gastrodia elata Bl. 结果:除引物对16外均能很好地区分野生天麻、无性繁殖天麻及有性繁殖天麻,其中有性繁殖天麻均扩增出2条带,为杂合子;
- The DNA from 25 rice varieties were amplified using 75 SSR primer pairs on 12 chromosomes in rice. 25 SSR pairs could be screened in 25 rice varieties. 摘要选用分布于水稻12条染色体上的75对SSR引物,对25个水稻品种进行DNA扩增,筛选到特异扩增条带的SSR引物25对。
- This study showed that the seven primer pairs of microsatellite locis screened were suitable for genetic diversity analysis of mud carp. 以上结果表明,筛选获得的7个微卫星座位适于对鲮进行遗传多样性分析。
- One marker, named as Nsp4, was obtained with the primer pair A3B6, whose genetic distance to SSN resistance gene was 8.73 cM. 引物组合A_3B_6找到了1个标记;命名为Nsp4;与抗茎线虫基因的遗传距离为8.;73cM。
- The AFLP primer pairs become useful tools for selecting the lowest somaclonal variation from the regenerated shoots of A. formosanus under the tissue culture program. 这些具有侦测力的AFLP引物组,将是筛选台湾金线连组织培养之最低体细胞变异分生苗的有利工具。
- E-ACT/M-CAC, E-AAC/M-CAA 及 E-AAG/M-CAA were screened out.They were the minimum number of primer pairs to be used to distinguish the 15 analyzed rice varieties. 通过优选,筛选出3对引物,即E-ACT/M-CAC,E-AAC/M-CAA及E-AAG/M-CAA,它们是本研究中用来区分所研究的15个材料所需的最少引物组合。
- Primer pairs C1/C2, D1/D2 and G1/G2 amplified different fragments from several genomic DNA samples. Further study should be done to improved PCR conditions or search for other specific primers for the detection of Cur. 引物C1/C2、D1/D2和G1/G2的扩增结果:来自于Cur.
- Samples used in this study were from 20 cases of EHT and NT respectively. Entire mtDNA 16.6kb extracted from their peripheral blood was amplified using 32 overlapping primer pairs. 在本研究中包括正常血压人群及原发性高血压病患者各20例,提取外周血液DNA,采用32对交叉重叠引物,PCR扩增全长线粒体16.;6kb基因。
- Using Specific Primer pair to Detect Bursaphelenchus xylophilus 应用特异引物组合检测松材线虫
- RC1(3+4), ALC(5)141 and LC1(5)57-4 deriving from 9248-6 lost one band based on the AFLP primer pair, 92S08/92G08. 4. The Pot2-PCR based fingerprints showed that lineage closely related to the cultivar with given R-genes. 92S08/92G08 引物对揭示3 个来自9248-6的菌株与其亲本菌株相比,缺失1 条带。
- Education should not be restricted to any one specific age group. 教育不应限制在任何特定的年龄组上。
- A total of 181 clear and repeatable bands were amplified from 27 selected RAPD primers, and 101 alleles were detected from 29 SSR primer pairs. 27个筛选的RAPD引物扩增出181条清晰可重复的带,29对SSR引物探测出101个位点。