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- signal peptides prediction 信号肽分类预测
- Signal peptides' structures and functions, signal sequence traps and the proteins expressions in prokaryote and eukaryote expression systems were illustrated in the article. 就信号肽的结构和功能,信号肽的捕获方法及其在原核表达系统和真核表达系统中表达外源蛋白质的应用做一些介绍。
- The Tat signal peptide detecting vector of PGA were constructed. 构建了Tat信号肽检测载体。
- We add the 5'AOX1 primer and 3'AOX1 primer amplify pPIC9K and alpha-factor signal peptide sequence was obtained. 考虑到克隆方便,在序列5’端和3’端分别引入BamHI和EcoRI 位点,并在两个酶切位点的外侧分别加上保护碱基。
- Once across the membrane, the signal peptide is cleaved by the signal peptidase and the process protein is released. 信号肽是疏水的,与膜上结合的蛋白接近,拉着后面的亲水多肽跨膜,一旦跨过膜,即被信号肽酶切除,释放出加工的蛋白。
- The location of the signal peptide of this peptide chain is 1-26, the breaking point is between 26 and 27 (GAA-AF). 应用神经网络算法和隐马氏模型分析结果显示该肽链信号肽位置为1-26,断裂点位置是26-27(GAA-AF)。
- The extramembrane region consists of 758 aminoacids, including signal peptide containing 31 amino acids at N-terminus and seven Ig-like domains. 胞外区由758个氨基酸组成,包括N端31个氨基酸的信号肽和七个类似免疫球蛋白(Ig)的结构域。
- There were no signal peptide and N-glycosylation sites, while had 4 conservative Cys amino acid in the deduced amino acid sequence. 在推导的猫IL-18氨基酸序列中,无信号肽序列和潜在的N-联糖基化位点,但存在4个Cys残基。
- The signal peptide at the N- termination can lead the protein to the periplasm.Six histidines at the C- termination will let the purification easy. 在质粒pET-20b(+)中,重组的蛋白会在N-末端带有一个信号肽序列,它可以使重组蛋白分泌到细胞的周质空间处,而其在C-末端还有带有6个组氨酸的标签,这样就使得重组蛋白的分离纯化更加的方便,简单。
- Proteins secreted by one step into the periplasm or through Bayer's junctions to the extracellular environment have a signal peptide. 蛋白可直接分泌到周质中,或通过Bayer接点分泌到胞外环境中的蛋白有一个信号肽。
- In this paper, signal peptide was added before the N-terminus of CEMA with the intention to reduce its toxic to plant cells while maintaining the strong antibacterial activity. 本文通过在CEMA的N端添加信号肽,对其进行改造,以探索不降低抑菌作用的同时,降低其对植物细胞毒害作用的可能性。
- GliTd-3 and GliTd-4 were both 861bp, and their deduced amino acid sequences were both 267 residues with a 19-residue putative signal peptide (absent from mature proteins). 而GliTd-1和GliTd-2编码区分别长821bp和859bp,由于编码区内存在提前终止密码子和插入/缺失引起的移码突变,推测其为不表达的假基因。
- With the signal peptide analysis software of the TMHMM2.0(http://genome.cbs.dtu.dk/services /TMHMM-2.0), the preceding peptide of Vip3A-WB5 was a transmembrane peptide. 通过TMHMM2.;0(http://genome
- Intercellular signaling peptides and proteins 胞间信号肽类和蛋白质类
- A red light is usually a signal of danger. 红灯通常是危险的信号。
- Intercellhlar signaling peptides and proteins 胞间信号肽类和蛋白质类
- Fibroblast growth factor(FGF-1)lacks amino-terminal signal peptide, so it can’t release through the classical endoplasmic reticulum(ER)-Golgi pathway. 酸性成纤维生长因子-1(FGF-1)由于氨基端缺乏信号肽序列,不能通过经典的内质网-高尔基体途径释放。
- Intere.eunlar signaling peptides and proteins 胞间信号肽类和蛋白质类
- With common molecular biology technique,we cloned SCF gene including signal peptide coding sequence coded signal peptide and constructed its expression plasmid. 采用常规的分子生物学技术,从小鼠骨髓细胞中克隆了含信号肽序列的SCF,并构建了表达载体。
- This article reviews signal peptide sequence about its structural feature,the common used signal peptide and the construction of vector with high secretory. 本文将从信号肽结构特征、常用的信号肽序列以及构建高分泌型载体等方面进行综述。