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- We add the 5'AOX1 primer and 3'AOX1 primer amplify pPIC9K and alpha-factor signal peptide sequence was obtained. 考虑到克隆方便,在序列5’端和3’端分别引入BamHI和EcoRI 位点,并在两个酶切位点的外侧分别加上保护碱基。
- This article reviews signal peptide sequence about its structural feature,the common used signal peptide and the construction of vector with high secretory. 本文将从信号肽结构特征、常用的信号肽序列以及构建高分泌型载体等方面进行综述。
- Signal Peptide Sequence and its Application to Protein Expression 信号肽序列及其在蛋白质表达中的应用
- signal peptide sequence 信号肽顺序
- There were no signal peptide and N-glycosylation sites, while had 4 conservative Cys amino acid in the deduced amino acid sequence. 在推导的猫IL-18氨基酸序列中,无信号肽序列和潜在的N-联糖基化位点,但存在4个Cys残基。
- With common molecular biology technique,we cloned SCF gene including signal peptide coding sequence coded signal peptide and constructed its expression plasmid. 采用常规的分子生物学技术,从小鼠骨髓细胞中克隆了含信号肽序列的SCF,并构建了表达载体。
- The Tat signal peptide detecting vector of PGA were constructed. 构建了Tat信号肽检测载体。
- The signal peptide, KDEL and E tag sequence was successfully introduced into 9.1C3 ScFv, and the intrabody gene was inserted into eukaryotic expression vector which can be expressed in COS7 cells. DNA序列测定证明 ,通过PCR成功地为 9 1C3ScFv引入了内质网定位所需的信号肽、KDEL及E tag序列 ,成功地构建了胞内抗体真核表达载体 ,通过胞内染色方法和Westernblot证明胞内抗体在COS7中得到表达。
- Sequence analysis of Gal-4 eDNA showed that the cDNA of Gal-4 gene con- sists of 321 bp,encoding Gal 4 polypeptide of 63 amino acids,which comprised of signal peptide with 20 amino acids at N'terminus,and mature peptide with 38 amino acids. 序列分析结果表明,获得的广西黄鸡Gal-4基因大小为321 bp,推导的Gal-4由63个氨基酸组成,其中N端20个氨基酸为信号肽,C端38个氨基酸组成Gal-4的成熟肽。
- 3b Nucleotide sequences and the deduced animo acid of the gene gN(UL49.5)of PRV strain Ea.The putative signal peptide sequences was under-lined,ripple indicated the transmembrance domains. 标题: 图3b PRV鄂A株gN(UL49.;5)基因核苷酸序列及其推导的氨基酸下画线表示信号肽序列;波浪线表示可能的跨膜区 Fig
- The sequence encodes the amino acids of a peptide sequence (the homeodomain) that enables the parent protein to bind to DNA. 另外同源框编码了一些多肽的氨基酸序列(同源异形域),这些多肽可使带有同源异形域的蛋白与DNA结合。
- The ZFP28 cDNA sequence is 4104bp and contains a 2076-bp open reading frame (ORF) that encodes an 868 amino acid protein with an N-terminal signal peptide, two KRAB domains and 14 C-terminal C2H2 zinc finger motifs. ZFP28长4104个碱基,含有一个长为2076个碱基的开放阅读框,编码一个长为868个氨基酸的蛋白质,它包含一个N-端信号肽,两个KRAB结构域和14个C-端C2H2型锌指结构。
- Once across the membrane, the signal peptide is cleaved by the signal peptidase and the process protein is released. 信号肽是疏水的,与膜上结合的蛋白接近,拉着后面的亲水多肽跨膜,一旦跨过膜,即被信号肽酶切除,释放出加工的蛋白。
- The location of the signal peptide of this peptide chain is 1-26, the breaking point is between 26 and 27 (GAA-AF). 应用神经网络算法和隐马氏模型分析结果显示该肽链信号肽位置为1-26,断裂点位置是26-27(GAA-AF)。
- Then, some of the positive phages were sequenced and their corresponding peptide sequence was deduced according to their DNA sequence. 经随机抽取部分阳性克隆进行DNA序列分析,推导出相应噬菌体呈现肽的氨基酸序列,通过序列比较分析相对保守的表位信息;
- The extramembrane region consists of 758 aminoacids, including signal peptide containing 31 amino acids at N-terminus and seven Ig-like domains. 胞外区由758个氨基酸组成,包括N端31个氨基酸的信号肽和七个类似免疫球蛋白(Ig)的结构域。
- The signal peptide at the N- termination can lead the protein to the periplasm.Six histidines at the C- termination will let the purification easy. 在质粒pET-20b(+)中,重组的蛋白会在N-末端带有一个信号肽序列,它可以使重组蛋白分泌到细胞的周质空间处,而其在C-末端还有带有6个组氨酸的标签,这样就使得重组蛋白的分离纯化更加的方便,简单。
- Proteins secreted by one step into the periplasm or through Bayer's junctions to the extracellular environment have a signal peptide. 蛋白可直接分泌到周质中,或通过Bayer接点分泌到胞外环境中的蛋白有一个信号肽。
- Signal peptides' structures and functions, signal sequence traps and the proteins expressions in prokaryote and eukaryote expression systems were illustrated in the article. 就信号肽的结构和功能,信号肽的捕获方法及其在原核表达系统和真核表达系统中表达外源蛋白质的应用做一些介绍。
- The binding of antigenic peptide sequences to major histocompatibility complex(MHC) molecules is a prerequisite for stimulation of cytotoxic T cell responses. 抗原多肽序列与主要组织相容性复合体(MHC)分子的结合是引起T细胞免疫应答的先决条件。