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- real - time quantitative RT - PCR 实时定量RT-PCR
- Methods A real time quantitative PCR(RQ-PCR) was performed to measure the bcr/abl~(P190) transcripts in the bone marrow mononuclear cells from 74 CML patients in different phases. 方法应用实时定量聚合酶链反应(RQ-PCR)检测74例CML患者骨髓单个核细胞中的bcr/ablP190转录本。
- Objective To construct and evaluate the competitive quantitative RT PCR method for detecting the fusion gene of AML1 ETO in the patients of t(8;21)AML(acute myeloid leukemia). 目的 建立针对 AML- ETO融合基因转录本的竞争性定量反向 -聚合酶链反应 (RT- PCR)体系 ,并探讨其在 t(8;2 1)急性髓细胞性白血病 (acute myeloid leukemia,AML)微小残留病变跟踪中的应用价值。
- The mRNA levels of this gene were determined in SCLC cell line SH77 ,big cell lung cancer cell line H460,lung adenocarcinoma cell line SPC A 1 and SPC A 1/CDDP using semi quantitative RT PCR assay. 应用半定量RT-PCR方法检验小细胞肺癌SH77、大细胞肺癌H460、肺腺癌SPC-A-1和SPC-A-1/cDDP等细胞株中该基因mRNA的表达。
- Keywords real time quantitative PCR;Roundup Ready soybean;GMO detection; 荧光定量PCR;转基因大豆;转基因检测;
- The copy number of the gene in chromosome of strain detected by the real time quantitative PCR 荧光定量PCR检测细菌染色体上基因拷贝数
- Rapid Molecular Detection of Down Syndrome by Real Time Quantitative Fluorescence PCR 实时荧光定量PCR快速分子诊断唐氏综合征
- Keywords coinfection;Borrelia burgdorferi;Anaplasma phagocytophilum;Lyme disease;real time quantitative PCR; 混合感染;伯氏螺旋体;嗜吞噬细胞无浆体;莱姆病;实时荧光定量PCR;
- Keywords Co-infection;Borrelia burgdorferi;Anaplasma phagocytophilum;Lyme disease;Real time quantitative PCR; 混合感染;伯氏疏螺旋体;嗜吞噬细胞无浆体;莱姆病;细胞因子;
- Real - time fluorescence quantitative RT - PCR 实时荧光定量PCR
- Keywords Real Time Quantitative PCR;chronic myelogenous leukemia;imatinib mesylate;BCR-ABL fusion gene;resistance mechanism; 实时定量PCR;慢性髓细胞白血病;伊马替尼;BCR-ABL融合基因;耐药机制;
- real time quantitative PCR 实时荧光定量PCR
- RT PCR was performed for APN gene expression in liver tissue. (2 )APN表达 :根据兔APN基因cDNA序列设计引物 ,提取肝总RNA ,利用RT PCR方法 ,检测肝APNmRNA水平的变化。
- Methods Plasma ETA was measured quantitively by RT PCR. 方法 应用 RT-PCR技术检测外周血循环中 ETA基因表达水平。
- The levels of Prx and TrxR mRNA were determined 24 h and 48 h post,transfection by relative quantitative RT,PCR,and the growth of Trichomonas vaginalis was estimated under microscope 36 h post,transfection. 收集转染前、转染后24 h和48 h的毛滴虫细胞,用半定量反转录-PCR(RT-PCR)检测Prx和TrxR的mRNA水平;
- Angiotensin receptor (ATR) mRNA expression of VSMC was measured by RT PCR. RT PCR检测VSMC血管紧张素受体 (ATR)mRNA表达。
- Expression of the M 2-1 gene was examined by RT PCR and Western blot. 重组真核质粒PXJ 4 1/M2 -1转染肺腺癌PAa细胞 ,应用RT PCR、Westernblot方法检测表达。
- Keywords senescence-accelerated mouse;learning and memory;Alzheimer s disease;cDNA microarray;real time Quantitative fluorescence RT-PCR;nootropic drug;gene target; 关键词快速老化模型小鼠;学习记忆;老年性痴呆;基因芯片;实时荧光定量PCR;益智药物;基因靶标;
- The expression of cyclin D1 and CDK4 were detected by RT PCR method. RT PCR检测细胞周期素D1(cyclinD1)、细胞周期蛋白依赖激酶 4 (CDK4 )mR NA的表达。
- This will be very quick. It will be real time. 它将是非常快捷和实时的。