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- Objective:To clone,prokaryotic express and purify APOBEC3G protein in vitro. 目的:原核表达重组APOBEC3G蛋白,为其功能及免疫原性研究奠定基础。
- The chimeric antigen produced by prokaryotic expressing system will be promisingly used to detect and diagnose AIDS in our country. 本论文利用原核表达体系制备的嵌合抗原有望用于我国艾滋病的检测与诊断；
- I have studied the technology of molecular biology, protein prokaryotic expressing, refolding in vitro, purification, identification and MHC tetramer. 我广泛查阅中外文文献，出色完成研究课题，为导师的国家自然科学基金及科技部973计划资助的课题奠定基础。
- Objective To construct the prokaryotic expression plasmid expressing woodchuck hepatitis virus core antigen(WHcAg) and prepare polyclonal antibodies. 目的构建土拨鼠肝炎病毒核心蛋白质粒并进行原核表达、抗体制备。
- The recombinant plasmid pMD-ORF2 was digested with EcoR V and Xhol I and then the fragment was sub-cloned into the prokaryotic expressing vector pET-32a(+), getting the recombinant plasmid pET-ORF2. 将重组质粒pMD-ORF2的EcoR V和Xhol I双酶切产物亚克隆到原核表达载体pET-32a(+)，构建ORF2基因重组表达质粒pET-ORF2。
- AIM: To construct pET32a/His MBL-CLR recombinant prokaryotic expression plasmid and to express mannan-binding lectin-CLR (MBL-CLR) protein in E. 目的:在大肠杆菌中表达人甘露聚糖结合凝集素(MBL)胶原样区(CLR)蛋白。
- Hai L,Wu K,Chen XG,et al.Cloning,prokaryotic express and immunoreactivity evaluation of Angiostrongylus Cantonensis galection[J].J South Med Univ/Nan Fang Yi Ke Da Xue Xue Bao,2007,27(5):584-7. [2]郝丽;吴馄;陈晓光;等.;广州管圆线虫半乳凝素基因的克隆表达、蛋白纯化及免疫反应性研究[J]
- Studies on Prokaryotic Expression of Plant Anti-freeze and Salt Tolerance Genes. 盐基因原核表达研究。
- The cDNA was subcloned into prokaryotic expression vector pET3d and overexpressed in E. coli BL21(DE3). 将该cDNA插入原核表达载体pET3d并在大肠杆菌BL21(DE3)中过量表达。
- Objective: 1.Subclone B7.2(IgV+C),IgV and IgC domains and construct prokaryotic expression system of them. 2. 目 的： 1．亚克隆B7.;2分子的胞外(IgV+C);IgV及IgC各区段并构建其原核表达载体。
- The study aimed to construct the prokaryotic expression vectors carrying MTB esat6 and lhp and lhp-esat6 fusion genes amplified by PCR, and express them in E. coli respectively. 本研究通过体外扩增MTB esat6、lhp及lhp-esat6融合基因，构建原核表达载体并在E.
- In addition, the metamer lectin expressed by prokaryotic expression system did not agglutinate rabbit red blood cells. 原核表达系统获得的凝集素亚基不能凝集兔血红细胞。
- Conclusion: The prokaryotic expression vector with target gene was constructed successfully. 结论:成功构建了带有目的基因的原核表达载体。
- Objective To clone the flagellar biosynthesis genes flhA,flhB_2 and fliR of Leptospira interrogans and construct their prokaryotic expression system. 目的克隆问号钩端螺旋体鞭毛相关基因flhA、flhB2和fliR,并构建其原核表达载体。
- Objective: To obtain the recombinant augiogenin (Ang) protein by means of prokaryotic expression system and investigate its bioactivity. 目的:利用原核表达系统获得重组血管生成素 (Ang)并研究其生物学活性。
- Full-length or truncated cDNA was subcloned into prokaryotic expression vector pET30a and expression induced in E. coli BL21(DE3). 全长的或C末端截短的鲨烯合酶cDNA被克隆进原核表达载体pET30a并在大肠杆菌BL21(DE3)中诱导表达。
- Methods:The epf gene fragment was amplified by PCR from ZYH24 and cloned into prokaryotic expression plasmid pGEX4T-2 to form pGEX4T-2-epf. 方法根据GenBank S.;suis2epf基因序列设计引物;克隆ZYH24株epf基因片段并进行序列分析;
- Then the BLY cDNA fragment was subcloned into prokaryotic expression vector pGEX-4T-1, forming the prokaryotic expression plasmid (pG-BLY). 克隆PCR产物，并构建了pGEX-4T-1-BLY原核表达载体。 经BamHI和EcoRI酶切及质粒PCR鉴定，证实本实验构建的新型牛溶菌酶基因已克隆到原核表达载体pGEX-4T-1上，为进一步研究其诱导表达条件及生物学功能奠定了基础。
- These results suggest that the prokaryotic expressed AGT protein is an effective immunogen for the preparation of anti-AGT antiserum . 以上结果提示，利用大肠杆菌融合表达的AGT蛋白可有效刺激家兔产生AGT抗体。
- Prokaryotic Expression of the Vacuolar Ca~(2+)/H~+ Antiporter SsCAX1 N-termi-nal and Its Polyclonal Antibody Preparation of Suaeda salsa L. 盐地碱蓬液泡膜Ca~(2+)/H~+逆转运蛋白SsCAX1 N末端原核表达和多克隆抗体的制备
