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oligo microarry 寡核苷酸微阵列技术
Oligo(dT)18 primer were from Sangon, Shanghai. Oligo(dT)18引物购自上海生工。
First , the Oligo dT-3sites Adaptor primer was used to conduct reverse transcription. 首先，以试剂盒提供的Oligo dT-3sites Adaptor primer为引物进行逆转录；
The transitions of bisphenol A and phenolphthalein oligo--polysulfones were inves-tigated by DSC. 本工作用示差扫描量热法(DSC)研究了双酚A型和酚酞型两种聚砜预聚物的转变。
The results showed that the preparation of the cultured oligo dendrocytes was mor... 本操作简单易行,可以在短时间内获得足量的纯化的少突胶质细胞。
The shortening is greater than 41%, the majority of which occurred in late Oligo cene and the minority in late Miocene. 缩短时期主要为渐新世末，其次为中新世末。
In the yeast two-hybrid screens, we used the EFP and eif5a domain of eIF5A as baits to screen an oligo(dT)-primed human mammary cDNA library. 在酵母双杂交实验中，我们分别以eIF5A的两个结构域EFP(N末端)和eif5a(C末端)为诱饵，在人类乳腺文库中筛选与其相互作用的蛋白质。
Oligo ethylene glycol diglycidyl ether was prepared from oligo ethylene glycol and epichlorohydrin in the presence of NaOH by PTC. 以多聚乙二醇和环氧氯丙烷为原料合成了多聚乙二醇二环氧甘油醚。
It is especially critical when labeling oligonucleotides, since even using pure dye results in multiple oligo conformers (6). 尤其是在标记寡聚核苷酸，即使是用纯的染料也会出现多个寡异构体。
The RNA was rerverse transcribed according to the illustration of cDNA first chain construction agent kit with random Primers Oligo dT. 由于其发病机理尚不完全清楚，早期诊断仍有困难。
In this article we review the principles and application of several types of biological techniques based on cellular pathoklisis including cell microarry,cell sensor and patch-clamp technique. 综述了细胞芯片、细胞传感器和膜片钳这几种基于细胞特异感受的生物学技术的原理及应用状况。
Once they seize hold, each tentacle begins to curl and extend, as the oligo squeezes the cytoplasm out of itself until only a cellophane-like sheet of membrane remains. 一旦被它抓住，每个触须就会卷曲和延伸。象少突细胞挤压细胞质那样直到变成一张象玻璃纸那样薄的隔膜。
Oligo microarray is used to perform parallel detection of 24 HCCs and their corresponding noncancerous liver tissues for the mutation types of p53 gene. 我们还利用寡核苷酸芯片技术并行检测了24 例肝癌及其相对的癌旁组织的样本中p53基因7 个常见突变位点。
Using the methods of phenol extraction and chromatography on oligo (dT)-cellulose, we have prepared human globin messenger RNA from blood obtained frompatients with neonatal jaundice due to ABO blood type incompatibility. 本文以新生儿ABO血型不配溶血换得的血网织红细胞为材料,用酚-氯仿提取及Oligo(dT)纤维素亲和层析制备了人珠蛋白mRNA。
Objective:To investigate the effects of EBV ?BHRF1? antisense oligo?deoxynucleotide (ASO) on the proliferation of poorly differentiated nasopharyngeal carcinoma (SUNE?1) cell lines. 目的：观察EB病毒BHRF1反义寡核苷酸片段对鼻咽癌SUNE?1细胞株增殖的影响。
The (oligo)saccharides were tagged with the charged fluorophore 8-aminonaphthalene-1,3,6-trisulfonate (ANTS), and the reductive amination reactions were essentially complete after approximately 16h under the given experimental conditions. 寡糖首先经8-氨基萘基-1,3,6-三磺酸(ANTS)胺化还原衍生,此反应过程在所给实验条件下需要16h。
Then an oligo nuclear hairpin sequence targeting TLR4 gene was designed by the internet tool siRNA Wizard and then inserted into the plasmid pEGFP-H1/siRNA so as to form the plasmid pEGFP-H1/TLR4-siRNA. 将其克隆入pEGFP-H1/siRNA,构建表达EGFP及TLR4-shRNA的真核表达质粒pEGFP-H1/TLR4-siRNA。
Oligo glucosamine was prepared by oxidative degradation of chitosan with hydroperoxide in the neutral condition.Its moisture re tention,hygroscopicity and antimicrobial activity were investigated. 采用双氧水在中性条件下对壳聚糖进行氧化降解,制备了低聚氨基葡萄糖。
It was found that the hydrogen bonded complexes of monomer template and oligo functional monomers before polymerization is the control factor governing the formation of microsphere. 研究了合成反应条件对聚合物形貌的影响,发现聚合前主客体氢键络合物和功能单体氢键低聚体是控制微球形成及其粒径大小的关键因素。
The peripheral blood taken before and after consuming low-sodium salt,were used to analyze the differences of the gene expression by Oligo Insulin Signaling Pathway SuperAarray,six chips in total. 研究低钠盐饮食对高血压与肥胖并发者血液中胰岛素信号通路相关基因表达的影响,初步找到表达发生变化的基因间相互关联的网络状态。