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- The RNA was rerverse transcribed according to the illustration of cDNA first chain construction agent kit with random Primers Oligo dT. 由于其发病机理尚不完全清楚,早期诊断仍有困难。
- Oligo(dT)18 primer were from Sangon, Shanghai. Oligo(dT)18引物购自上海生工。
- In this experiment, differential expressing genes in leg muscle of chicken mentioned above were studied via DDRT-PCR technique, which firstly adopted 72 primer pairs that piloted by 3 T7 (dT11) N as anchor primers and 24 M13-ARPs as random primers. 本实验采用3个26bp的T7(dT11)N为锚定引物,24个26bp的M13-ARPs为随机引物,组成72个引物组合对上述四个品种雏鸡腿肌组织进行DDRT-PCR反应。
- Reagents I used anchored primer - T7( dT12) Aps and stochastic primer - M13r - ARPs in my experience, the reagent of distilling and RNA produced by IBCOBRLG company. 实验试剂:本实验应用锚定引物T7(dT12)APs和随机引物M13r一ARPs。 RNA提取(TRIZOL)、逆转录试剂盒为GIBCOBRL公司产品;
- The anchor man in a relay team runs last. 接力队中的主力跑最后一棒。
- I borrowed from the library a primer of phonetics. 我从图书馆借了一本语音学入门书。
- Several boats lay at anchor in the harbour. 港湾里停泊著几只船。
- The anchor chain took charge and ran out. 锚链失去控制而滑了出来。
- The ship lay at anchor in the bay. 这艘船停泊在海湾。
- The ships cast anchor because of the heavy fog. 船因浓雾拋锚。
- The ship cast anchor at Shanghai. 这船在上海下锚停泊。
- They weighed anchor and drifted toward shore. 他们起了锚,向海岸漂去。
- We lay at anchor outside the harbour. 我们在港外抛锚停泊。
- Form where we lay anchor, we could see the ships in the offing. 从我们抛锚的地方可以看到近海的船。
- First , the Oligo dT-3sites Adaptor primer was used to conduct reverse transcription. 首先,以试剂盒提供的Oligo dT-3sites Adaptor primer为引物进行逆转录;
- In the yeast two-hybrid screens, we used the EFP and eif5a domain of eIF5A as baits to screen an oligo(dT)-primed human mammary cDNA library. 在酵母双杂交实验中,我们分别以eIF5A的两个结构域EFP(N末端)和eif5a(C末端)为诱饵,在人类乳腺文库中筛选与其相互作用的蛋白质。
- Somehow or other he always comes to an anchor in the bar. 不知怎么地,他老是泡在酒吧里。
- They brought the boat into the harbour and dropped (the) anchor. 他们把船开进海港下锚停泊。
- A coat of primer should be made over the bare surface before the main painting. 在喷涂主漆前,应在物品的表面刷一层底漆。
- The anchor line fouled on a rock. 锚索缠在一块岩石上