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Isoelectric focusing may be used as another indication. 等电聚焦可以用为另一指标。
Methods: Isoelectric focusing in polyacylamide gels was used. 用薄层聚丙烯酰胺凝胶等电聚焦的方法。
After quantification, the protein samples were separated by isoelectric focusing electrophoresis and then by SDS-PAGE. 定量后，分别进行一维等电聚焦分离和二维聚丙烯酰胺凝胶电泳分离。
Isoelectric focusing A technique used in ELECTROPHORESIS to separate amphoteric molecules (able to combine with either acids or bases). 等电点聚焦：是一种利用电泳技术分离两性分子（能与酸或碱结合）的技术。
The variant(s) most likely to show pI values different from that of HbA when run on an isoelectric focusing gel. 在跑IEF时最有可能表现出与HbA不同pI的变种。
Simultaneous Isoelectric Focusing of EsD EAP ADA on Uitrathin-layer Polyacrylamide Gels. 非平衡超薄层等电聚焦同步检测EsD,EAP,ADA同功酶
UTLIEF(Ultrathin-layer isoelectric focusing) was used for isozyme analysis of root-knot nematodes for the first time. 3．首次应用超薄等电聚焦电泳技术对30个根结线虫种群酯酶同功酶进行成功分析。
Whitmore.Electrophoretic and isoelectric focusing technique in fisheries management[M].Boston:CPC Press,1990.28-30. 胡能书;万贤国同工酶技术及其应用[M].;长沙:湖南科学技术出版社;198573-85
After being concentrated and desalted, the samples were separated by isoelectric focusing on first dimension and SDS electrophoresis on second dimension. 样品浓缩除盐后，进行一维等电聚焦分离和二维SDS电泳分离提取人肾组织磷酸化蛋白。
Methods:ALP isoenzymes in the serum of normal persons and tumor patients had been separated by agarose isoelectric focusing(IEF). 方法:采用神经氨酸苷酶对正常人及患者血清中ALP同工酶进行处理后进行琼脂糖等电聚焦。
The component and isoelectric point of all parts of the sample were deter- mined by capillary isoelectric focusing (CIEF). 经毛细管等电聚焦电泳(CIEF)的方法测定其组成及各组分的等电点。
Methods The protein was purified by common polyacrylamide gel electrophoresis (PAGE), isoelectric focusing electrophoresis (IEF) and isotachophoresis (ITP). 方法用普通聚丙烯酰胺凝胶提纯电泳(PAGE)、等电聚焦提纯电泳(IEF)和等速提纯电泳(ITP)对样品进行分离。
Methods:In CSF and serum from 398 patients with neurological diseases ,51 of whom had MS,OCB were detected using isoelectric focusing with immunofixation. 方法:收集398例神经系统疾患的脑脊液和血清标本,其中51例MS患者,用等电聚焦加免疫标记的方法进行OCB的检测。
RESULTS: Compared with the recommended condition, the profiles were improved by enhancing the voltage of isoelectric focusing, extending the time and desalinizing the samples. 结果：提高聚焦电压增强聚焦及脱盐处理可获得比推荐条件更好的双向电泳图谱。
In this study, isoelectric focusing electrophoresis program was optimized, and various sample preparation methods, different loading quantities and strip length were studied. 在实验中，优化了等电聚焦程序，研究了精子蛋白的不同制备方法、不同上样量、不同胶条长度对电泳结果的影响。
The separation modes include capillary zone electrophoresis(CZE),micellar electrokinetic chroma-tography(MEKC),isoelectric focusing(IEF) and open channel electrochromatography(OCEC). 分离模式包括毛细管区带电泳(CZE),胶束电动色谱(MEKC),等电聚集(IEF),开管电色谱(OCEC)等。
Esterase isozyme upon isoelectric focusing eletrophores demonstrated that the high levels of total esterase activity in ABM R was due to the increased activities of E7, E13 and E15 isozymes. 从酯酶同工酶等电聚焦电泳得出 ,ABM- R种群的 E7、E13和 E15同工酶活力显著提高是导致 ABM- R种群酯酶活力提高的主要原因。
On line coupling of capillary isoelectric focusing (CIEF) to electrospray ionization mass spectrometry (ESI MS) as a two dimensional separation/analysis system was employed for high resolution analysis of the protein isoforms observed during CIEF process. 在线的毛细管等电聚焦电喷雾质谱联用 ,作为一种二维的分离系统 ,对毛细管等电聚焦过程中形成的蛋白质亚型进行了分析。
The separation modes include capillary zone electrophoresis(CZE), micellar electrokinetic chromatography (MEKC), isoelectric focusing (IEF) and open channel electrochromatography (OCEC). 分离模式包括毛细管区带电泳(CZE)，胶束电动色谱(MEKC)，等电聚集(IEF)，开管电色谱(OCEC)等。
The 16 given wild rice species belong to the genomes AA, BB, CC, CCDD, BBCC, EE, or FF. The results of esterase isozymes isoelectric focusing electrophoresis indicate that there was individually a special pattern of esterase among Ser. sativae, Ser. 对16个野生稻种(基因组分别为AA、BB、CC、CCDD、BBCC、EE和FF)的酯酶等电聚焦电泳分析表明:普通野生稻组的普通野生稻系和宽叶野生稻系、狭叶野生稻组的酯酶酶谱各有其特定模式,这和传统的稻属分类结果相一致。